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1. WO2007137700 - MUTATED DNA-POLYMERASES WITH HIGHER SELECTIVITY

Publication Number WO/2007/137700
Publication Date 06.12.2007
International Application No. PCT/EP2007/004315
International Filing Date 15.05.2007
IPC
C12N 15/11 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
C12N 9/12 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
CPC
C12N 9/1252
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
1241Nucleotidyltransferases (2.7.7)
1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Applicants
  • UNIVERSITÄT KONSTANZ [DE]/[DE] (AllExceptUS)
  • MARX, Andreas [DE]/[DE] (UsOnly)
  • RUDINGER, Nicolas [DE]/[DE] (UsOnly)
Inventors
  • MARX, Andreas
  • RUDINGER, Nicolas
Agents
  • PERREY, Ralf
Priority Data
10 2006 025 153.930.05.2006DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) MUTIERTE DNA-POLYMERASEN MIT HÖHERER SELEKTIVITÄT
(EN) MUTATED DNA-POLYMERASES WITH HIGHER SELECTIVITY
(FR) ADN POLYMÉRASES MUTANTES À SÉLECTIVITÉ ÉLEVÉE
Abstract
(DE)
Die vorliegende Erfindung betrifft eine DNA-Polymerase mit erhöhter Fehlpaarungsdiskriminierung, welche im Vergleich zur Wildtyp-Variante mindestens eine Mutation in ihrer Aminosäure-Sequenz aufweist, sowie eine Wirtszelle und ein Verfahren zur Herstellung der DNA-Polymerase. Desweiteren betrifft die vorliegende Erfindung die Verwendung der DNA-Polymerase in diagnostischen und molekularbiologischen Verfahren und ein Kit, welches die DNA-Polymerase enthält.
(EN)
The invention relates to a DNA-polymerase with increased mismatch discrimination which, compared to wild type variants, has at least one mutation in the amino acid sequence thereof, also to a host cell and to a method for producing the DNA-polymerase. The invention also relates to the use of the DNA-polymerase in diagnostic and molecular biological methods, and to a kit which contains the DNA-polymerase.
(FR)
L'invention concerne une ADN polymérase présentant une discimination de mésappariements accrue qui, comparativement aux variantes de type sauvage, présente au moins une mutation dans sa séquence aminoacide, ainsi qu'une cellule hôte, et un procédé de production de ladite ADN polymérase. L'invention concerne en outre l'utilisation de l'ADN polymérase dans des procédés de diagnostic et de biologie moléculaire, ainsi qu'un kit renfermant l'ADN polymérase.
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