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1. (WO2007127999) USE OF PRODUCTS OF PCR AMPLIFICATION CARRYING ELEMENTS OF SECONDARY STRUCTURE TO IMPROVE PCR-BASED NUCLEIC ACID DETECTION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/127999 International Application No.: PCT/US2007/067836
Publication Date: 08.11.2007 International Filing Date: 30.04.2007
IPC:
C12Q 1/68 (2006.01) ,C12P 19/34 (2006.01) ,C07H 21/04 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
Applicants:
KUTYAVIN, Igor [RU/US]; US
Inventors:
KUTYAVIN, Igor; US
Agent:
DAVISON, Barry, L. ; 2600 Century Square 1501 Fourth Avenue Seattle, Washington 98101-1688, US
Priority Data:
60/795,70528.04.2006US
Title (EN) USE OF PRODUCTS OF PCR AMPLIFICATION CARRYING ELEMENTS OF SECONDARY STRUCTURE TO IMPROVE PCR-BASED NUCLEIC ACID DETECTION
(FR) UTILISATION DE PRODUITS D'AMPLIFICATION PCR PORTANT DES ÉLÉMENTS DE STRUCTURE SECONDAIRE POUR AMÉLIORER LA DÉTECTION D'ACIDES NUCLÉIQUES PAR PCR
Abstract:
(EN) Particular aspects comprise amplifying target nucleic acid using PCR and an oligonucleotide primer pair wherein at least one of the primers is designed to incorporate a 5'-specialty sequence to provide for an amplification product that intramolecularly folds into a secondary structure; and detecting the amplification product by a method comprising: providing an oligonucleotide cleavage component, hybridizing said oligonucleotide cleavage component with the amplification product to form a three-strand cleavage structure wherein two strands of the three-strand cleavage structure are provided by the secondary structure of the amplification product, cleaving 3'- or 5'-strands of the three-strand cleavage structure using a duplex-specific nuclease activity resulting in a cleavage product, and detecting the cleavage product indicative of the presence of the target nucleic acid. In certain aspects both primers incorporate a 5'-specialty sequence and detecting comprises cleaving 3'- or 5'-strands of a three-strand cleavage structure using duplex-specific nuclease to provide a cleavage product.
(FR) Certains aspects de l'invention concernent l'amplification d'un acide nucléique par réaction en chaîne de la polymérase (PCR) et une paire d'amorces oligonucléotidiques, l'une au moins des amorces étant conçue pour incorporer une séquence de spécialité 5'et fournir un produit d'amplification à repli intramoléculaire dans une seconde structure; et la détection du produit d'amplification selon une méthode consistant: à prendre un composant de clivage oligonucléotidique, à hybrider ledit produit de clivage avec le produit d'amplification pour former une structure de clivage à trois brins - dont deux sont fournis par la structure secondaire du produit d'amplification - à cliver les brins 3' ou 5' de la structure de clivage à trois brins au moyen d'une activité nucléase spécifique de double hélice dont le résultat est un produit de clivage, et à détecter le produit de clivage indiquant la présence de l'acide nucléique cible. Selon certains aspects, les deux amorces intégrent une séquence de spécialité 5'. La détection consiste à cliver des brins 3' ou 5' de la structure de clivage à trois brins au moyen d'une nucléase spécifique de double hélice pour obtenir un produit de clivage.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP2021506US20100143898