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1. (WO2007124478) METHOD AND SYSTEM FOR PREPARING SOFT TISSUE FOR GRAFTING, ENHANCING GRAFTING RESULTS, AND GRAFTING AUTOLOGOUS FAT AND ADIPOCYTE DERIVED STEM CELLS TO SOFT TISSUE SUCH AS THE BREAST AND OTHER TISSUE DEFECTS
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What is Claimed Is:
1) A method for augmenting a soft tissue site of a subject, comprising the steps of:
a) applying a distractive force to said soft tissue site;
b) obtaining a sample of fat; and
c) injecting at least a portion of said sample of fat from step (b) into said soft tissue site that was subjected to a distractive force;
thereby augmenting the soft tissue site of said subject.

2) The method of Claim 1 , wherein the distractive force is continuously applied in step (a) for at least 24 hours prior to performing step (c).

3) The method of Claim 2, wherein the distractive force is intermittently applied in step (a) for at least 10 hours per day for about 28 days prior to performing step (c).

4) The method of claim 1, wherein the distractive force of step (a) is an externally applied mechanical force.

5) The method of claim 4, wherein said soft tissue site is at least one human breast and the distractive force is applied in step (a) with a device comprising a dome which fits over said at least one breast, a rim that seals said dome around said breast, and a pump for creating a vacuum within said dome.

6) The method of claim 5, wherein said rim has a sticky surface adapted to adhere to the patient's skin to thereby create and hold a pressure seal between said dome and said patient.

7) The method of claim 5, wherein said pump is a manual pump.

8) The method of claim 5, wherein said pump further comprises an independent power source and a pressure sensor.

9) The method of claim 8, wherein said pump is continuously engaged with said device to maintain a constant vacuum pressure inside said dome.

10) The method of claim 9, wherein said constant vacuum pressure is maintained between about 20 to 25 mm of Hg.

11) The method of claim 1, wherein steps (a) and (c) are performed contemporaneously on two breasts of a human subject.

12) The method of claim 11 , wherein both breasts are subjected to a similar vacuum pressure with a device comprising two domes which fit over said breasts, two rims that seal said domes around said breasts, a pump, and a conduit that places said pump in fluid communication with each of said domes.

13) The method of claim 12, wherein one breast is subjected to a different vacuum pressure than the other breast in step (a) with a device comprising two domes which fit over the breasts of said subject, a rim that seals each of said domes around each of said breasts, and a separate pump for each of said domes.

14) The method of claim 1, wherein said distractive force is applied with a device which mechanically attaches to the soft tissue site and induces a distractive force in the underlying soft tissue site.

15) The method of claim 14 wherein said distractive force is applied with a device positioned internally to said soft tissue site.

16) The method of claim 1 , wherein said sample of fat is removed with a syringe from at least one of an abdominal region, a thigh region, a flank region, a gluteal region, or a trochanter of said subject.

17) The method of claim 16, wherein said syringe is approximately a 10 ml syringe fitted with a 14 gauge cannula.

18) The method of claim 1 , further comprising separating said sample of fat from serum and tumescent fluid prior to step (c).

19) The method of claim 18, wherein said sample of fat is separated from serum and tumescent fluid by decanting the fluid from the fat.

20) The method of Claim 1 , wherein said soft tissue is injected with said sample of fat in step (c) with a syringe.

21) The method of claim 20, wherein said syringe is a 10-50 ml syringe fitted with a 14 gauge cannula (2-3 mm).

22) The method of claim 1 , wherein said injections of step (c) are into a subcutaneous plane of a breast.

23) The method of claim 1 , wherein said injections of step (c) are into a deep
retroglandular space of a breast.

24) The method of claim 1, wherein said injections of step (c) are into both a deep retroglandular space and a subcutaneous plane of a breast.

25) The method of claim 1 , wherein said injections of step (c) are made in a three dimensional fanning pattern to micro-weave said sample of fat in a breast.

26) The method of claim 25, wherein said injections of step (c) are made in those regions of a breast not including a breast parenchyma region.

27) The method of claim 1 , wherein said injections of step (c) are made by injecting microdroplets of said fat sample throughout a breast.

28) The method of claim 1, wherein said soft tissue comprises at least one breast and further comprising applying a distractive force to said breast after it is injected at about 12 to 18 hours after completion of step (c).

i 29) The method of claim 28, wherein the distractive force is intermittently applied to said breast for at least about 6 to 22 hours per day for about 2 days after step (c).

30) The method of claim 29, wherein the distractive force is intermittently applied to said breast for at least about 10 hours per day for about 2 to 7 days after step (c).

31) A method of improving the survival of a fat graft into a targeted soft tissue site including applying a distractive force to said soft tissue site prior to the fat graft.

32) The method of claim 31 wherein the step of applying the distractive force includes applying at least intermittently a vacuum to said soft tissue site for at least 8 hours prior to grafting.

33) The method of claim 1 , wherein said sample of fat is obtained by a method comprising the steps of:
1 a) removing fat from said subject;
b) propagating said fat from step (a) outside of the subject;
c) and collecting the sample of fat formed in step (b).

34) The method of claim 1 , wherein said sample of fat is obtained by a method comprising the steps of:
a) removing at least one living cell from said subject;
b) propagating said cell or cells from step (a) outside of the subject under conditions where said cell or cells form a sample of fat;
c) and collecting the sample of fat formed in step (b) .

35) The method of claim 1, wherein said sample of fat is obtained from a source other than said subject.

36) The method of claim 35, wherein said source is a human.

37) The method of claim 36, wherein said human is genetically related to said subject.

38) The method of claim 36, wherein said human is a twin of the subject.

39) The method of claim 1 , wherein said source is a stem cell or cells.

40) The method of claim 39, wherein said stem cell or cells are propagated outside of the subject under conditions where said stem cell or cells form a sample of fat.

41) A method of preparing a soft tissue site for receiving a fat graft comprising identifying a portion of soft tissue targeted for receiving a fat graft, and applying a distractive force to said targeted soft tissue site for an extended time period prior to injecting a fat graft into said soft tissue site.

42) The method of claim 41 wherein applying said distractive force comprises applying an external mechanical force.

43) The method of claim 42 wherein applying said external mechanical force comprises applying a vacuum at least intermittently to said soft tissue site.

44) The method of claim 43 wherein applying said vacuum includes applying a vacuum for periods of time within about 10 hours per day for about four weeks prior to the fat grafting.

45) The method of claim 43 wherein applying said external mechanical force includes applying the distractive force substantially continuously for the 24 hours next preceding the injection of fat.

46) The method of claim 41 wherein the targeted soft tissue site includes a human breast, and applying a distractive force includes externally applying a vacuum.