WO2007120208 NANOGRID ROLLING CIRCLE DNA SEQUENCING

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Publication Number WO/2007/120208

Publication Date 25.10.2007

International Application No. PCT/US2006/044209

International Filing Date 14.11.2006

IPC

CPC

Applicants

PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US] (AllExceptUS)
CHURCH, George, M. [US]/[US] (UsOnly)
PORRECA, Gregory, J. [US]/[US] (UsOnly)
SHENDURE, Jay [US]/[US] (UsOnly)
ROSENBAUM, Abraham [US]/[US] (UsOnly)

Inventors

CHURCH, George, M.
PORRECA, Gregory, J.
SHENDURE, Jay
ROSENBAUM, Abraham

Agents

IWANICKI, John, P.

Priority Data

60/736,923

14.11.2005

Publication Language English (en)

Filing Language English (EN)

Designated States

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Title

(EN) NANOGRID ROLLING CIRCLE DNA SEQUENCING

(FR) SÉQUENÇAGE DE L'ADN EN CERCLE TOURNANT SUR NANOGRILLE

Abstract

(EN) The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

(FR) La présente invention concerne des procédés permettant le séquençage d'un polynucléotide immobilisé sur un réseau comportant une pluralité de régions spécifiques, possédant chacune un diamètre défini. Les procédés consistent à synthétiser un concatémère d'un polynucléotide par amplification en cercle tournant, le concatémère présentant un diamètre en coupe transversale supérieur au diamètre d'une région spécifique, à immobiliser le concatémère sur la région spécifique afin d'en faire un concatémère immobilisé et à séquencer ledit concatémère immobilisé.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68	involving nucleic acids

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68	involving nucleic acids
6869	Methods for sequencing
6874	involving nucleic acid arrays, e.g. sequencing by hybridisation

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q	MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1	Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68	involving nucleic acids
6869	Methods for sequencing
6874	involving nucleic acid arrays, e.g. sequencing by hybridisation

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1. WO2007120208 - NANOGRID ROLLING CIRCLE DNA SEQUENCING