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1. WO2007104019 - HYBRID PROTEIN WITH COX AND PGES ENZYME ACTIVITIES

Publication Number WO/2007/104019
Publication Date 13.09.2007
International Application No. PCT/US2007/063590
International Filing Date 08.03.2007
IPC
C12P 21/04 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
04Cyclic or bridged peptides or polypeptides, e.g. bacitracin
C12P 21/08 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
08Monoclonal antibodies
CPC
C07K 2319/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
C12N 9/0083
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0071acting on paired donors with incorporation of molecular oxygen (1.14)
0083Miscellaneous (1.14.99)
C12N 9/90
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
90Isomerases (5.)
C12Y 503/99002
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
503Intramolecular oxidoreductases (5.3)
99Other intramolecular oxidoreductases (5.3.99)
99002Prostaglandin-D synthase (5.3.99.2)
Applicants
  • BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM [US]/[US] (AE, AG, AL, AM, AT, AU, AZ, BA, BB, BE, BF, BG, BJ, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CM, CN, CO, CR, CU, CY, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IS, IT, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SI, SK, SL, SM, SN, SV, SY, SZ, TD, TG, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW)
  • RUAN, Ke-he [US]/[US] (UsOnly)
Inventors
  • RUAN, Ke-he
Agents
  • MINTZ, Carol, G.
Priority Data
60/780,18708.03.2006US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) HYBRID PROTEIN WITH COX AND PGES ENZYME ACTIVITIES
(FR) PROTÉINE HYBRIDE PRÉSENTANT LES ACTIVITÉS DES ENZYMES COX ET PGES
Abstract
(EN) A representative hybrid or engineered protein is constructed by linking together human cyclooxygenase (COX) isoform-2 (COX-2) and prostaglandin E2 synthase (PGES) via a 10-20 amino acid residues of a transmembrane sequence. The single protein molecule is expressed in cells, and adopts the functions of COX and PGES, to continually convert arachidonic acid (AA) into prostaglandin G2 (catalytic step 1), prostaglandin H2 (catalytic step 2) and prostaglandin E2 (PGE2; catalytic step 3). The COX-2-10aa-mPGES hybrid protein also converts dihomo-γ- linolenic acid (DGLA) into PGE1.
(FR) La protéine hybride mise au point décrite dans cette invention est construite par liaison de l'isoforme-2 (COX-2) de la cyclooxygénase humaine (COX) et de la prostanglandine E2 synthase (PGES) par l'intermédiaire de résidues d'acides aminés d'une séquence transmembranaire. Cette molécule de protéine unique est exprimée dans des cellules et elles adopte les fonctions des enzymes COX et PGES afin de convertir en continu l'acide arachidonique (AA) en prostaglandine G2 (étape catalytique 1), prostaglandine H2 (étape catalytique 2) et prostaglandine E2 (PGE2) (étape catalytique 3). La protéine hybride COX-2-10aa-mPGES convertit également l'acide dihomo-y-linolénique (DGLA) en PGE1.
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