WO2007104000 HYBRID PROTEIN THAT CONVERTS ARACHIDONIC ACID INTO PROSTACYCLIN

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Publication Number WO/2007/104000

Publication Date 13.09.2007

International Application No. PCT/US2007/063542

International Filing Date 08.03.2007

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Applicants

BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM [US]/[US] (AllExceptUS)
RUAN, Ke-he [US]/[US] (UsOnly)

Inventors

RUAN, Ke-he

Agents

MINTZ, Carol, G.

Priority Data

60/780,120

08.03.2006

Publication Language English (en)

Filing Language English (EN)

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Title

(EN) HYBRID PROTEIN THAT CONVERTS ARACHIDONIC ACID INTO PROSTACYCLIN

(FR) PROTÉINE HYBRIDE PERMETTANT DE TRANSFORMER L'ACIDE ARACHIDONIQUE EN PROSTACYCLINE

Abstract

(EN) A recombinant 130-kDa protein is constructed by linking together human cyclooxygenase (COX) isoform-2 (COX-2) and prostacyclin synthase (PGIS), via a 10-20 amino acid residues of a transmembrane sequence. The engineered protein is expressed in cells, and adopts the functions of COX and PGIS, to continually convert arachidonic acid (AA) into prostaglandin G2 (catalytic step 1), prostaglandin H2 (catalytic step 2) and prostacyclin (PGI2; catalytic step 3).

(FR) Cette invention concerne une protéine de recombinaison 130-kDa construite par liaison de l'isoforme-2 (COX-2) de la cyclooxygénase humaine (COX-2) et de la prostacycline synthase (PGIS), par l'intermédiaire de 10 à 20 résidus d'acides aminés d'une séquence transmembranaire. La protéine mise au point est exprimée dans des cellules et elle adopte les fonctions de COX et PGIS, afin de convertir en continu l'acide arachidonique (AA) en prostaglandine G2 (étape catalytique 1), prostaglandine H2 (étape catalytique 2) et prostacycline PGIS (étape catalytique 3).

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
02	having a known sequence of two or more amino acids, e.g. glutathione
04	Cyclic or bridged peptides or polypeptides, e.g. bacitracin

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
08	Monoclonal antibodies

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
K	PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39	Medicinal preparations containing antigens or antibodies

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
02	having a known sequence of two or more amino acids, e.g. glutathione
04	Cyclic or bridged peptides or polypeptides, e.g. bacitracin

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21	Preparation of peptides or proteins
08	Monoclonal antibodies

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1. WO2007104000 - HYBRID PROTEIN THAT CONVERTS ARACHIDONIC ACID INTO PROSTACYCLIN

C	CHEMISTRY; METALLURGY
07	ORGANIC CHEMISTRY
K	PEPTIDES
14	Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435	from animals; from humans
705	Receptors; Cell surface antigens; Cell surface determinants
72	for hormones
723	G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes; Proenzymes; Compositions thereof
0004	Oxidoreductases (1.)
0071	acting on paired donors with incorporation of molecular oxygen (1.14)
0083	Miscellaneous (1.14.99)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
114	Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
99	Miscellaneous (1.14.99)
99001	Prostaglandin-endoperoxide synthase (1.14.99.1), i.e. cyclooxygenase