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1. (WO2007103379) METHOD FOR ENDOTHELIAL CELL EXTRACTION FROM ADIPOSE TISSUES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/103379 International Application No.: PCT/US2007/005706
Publication Date: 13.09.2007 International Filing Date: 07.03.2007
IPC:
C12N 5/02 (2006.01) ,C12N 5/00 (2006.01) ,C12N 5/071 (2010.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02
Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
Applicants:
HUMACYTE [US/US]; P.O. Box 12016 Research Triangle Park, NC 27709, US (AllExceptUS)
NIKLASON, Laura, E. [US/US]; US (UsOnly)
LI, Yuling [US/CN]; US (UsOnly)
Inventors:
NIKLASON, Laura, E.; US
LI, Yuling; US
Agent:
KAGAN, Sarah, A.; Banner & Witcoff, Ltd. 1100 13th Street, N.W. Suite 1200 Washington, DC 20005-4051, US
Priority Data:
60/779,45407.03.2006US
Title (EN) METHOD FOR ENDOTHELIAL CELL EXTRACTION FROM ADIPOSE TISSUES
(FR) PROCEDE D'EXTRACTION DE CELLULES ENDOTHELIALES DE TISSUS ADIPEUX
Abstract:
(EN) Adipose tissue has proven to serve as an abundant, accessible, and rich source of endothelial or vascular endothelial cells suitable for tissue engineering. We describe a detailed method for the isolation and purification of endothelial cells using purified enzymes and antibody-based selection. The cells can be obtained from liposuction procedures and used in vascular grafts.
(FR) La présente invention concerne un tissu adipeux qui sert de source abondante, accessible et riche de cellules endothéliales ou vasculaires convenant pour l'ingénierie des tissus. Nous décrivons un procédé détaillé d'isolement et de purification de cellules endothéliales en utilisant des enzymes purifiées et une sélection à base d'anticorps. Les cellules peuvent être obtenues à partir de procédures de liposuccion et utilisées lors de greffes vasculaires.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1989290JP2009528841US20090319033CN101437937CA2644115AU2007223994
IN7634/DELNP/2008