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Machine translation
1. (WO2007091064) END MODIFICATION TO PREVENT OVER-REPRESENTATION OF FRAGMENTS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2007/091064    International Application No.:    PCT/GB2007/000427
Publication Date: 16.08.2007 International Filing Date: 07.02.2007
IPC:
C12N 15/10 (2006.01)
Applicants: SOLEXA LIMITED [GB/GB]; Chesterford Research Park, Little Chesterford, Saffron Walden Essex CB10 1XL (GB) (For All Designated States Except US).
RIGATTI, Roberto [IT/GB]; (GB) (For US Only).
GORMLEY, Niall, Anthony [IE/GB]; (GB) (For US Only).
BURGAR, Helen, Rachel [GB/GB]; (GB) (For US Only)
Inventors: RIGATTI, Roberto; (GB).
GORMLEY, Niall, Anthony; (GB).
BURGAR, Helen, Rachel; (GB)
Agent: WHITE, Nina, Louise; BOULT WADE TENNANT, Verulam Gardens, 70 Gray's Inn Road, London WC1X 8BT (GB)
Priority Data:
60/771,358 08.02.2006 US
Title (EN) END MODIFICATION TO PREVENT OVER-REPRESENTATION OF FRAGMENTS
(FR) MODIFICATION TERMINALE POUR EMPÊCHER LA SURREPRÉSENTATION DE FRAGMENTS
Abstract: front page image
(EN)The invention relates to a method of preparing a 5' and 3' modified library of template polynucleotides and also the use of the 5' and 3' modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5' and 3' modified library of template polynucleotides which have common sequences at their 5' ends and at their 3' ends, wherein over-representation of 'end' sequences of the primary polynucleotide molecules from whence the 5' and 3' modified library is generated is greatly reduced or prevented.
(FR)L'invention concerne un procédé de préparation d'une bibliothèque de polynucléotides matrices à modification 5' et 3' ainsi que l'utilisation de cette bibliothèque de matrice à modification 5' et 3' dans des procédés d'amplification d'acides nucléiques en phase solide. En particulier, l'invention concerne un procédé de préparation d'une bibliothèque de polynucléotides matrices à modification 5' et 3' qui possèdent des séquences communes à leurs extrémités 5' et à leurs extrémités 3', la surreprésentation des séquences 'terminales' des molécules polynucléotidiques primaires à partir desquelles la bibliothèque à modification 5' et 3' est générée étant fortement réduite ou empêchée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)