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1. (WO2007089093) PRODUCTION OF A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN BY THE SIGNAL SEQUENCE AND SECRETIONAL ENHANCER
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2007/089093    International Application No.:    PCT/KR2007/000515
Publication Date: 09.08.2007 International Filing Date: 30.01.2007
IPC:
C12N 15/63 (2006.01)
Applicants: REPUBLIC OF KOREA (REPUBLIC OF NATIONAL FISHERIES RESEARCH AND DEVELOPMENT INSTITUTE) [KR/KR]; 408-1 Sirang-ri, Gijang-eup, Gijang-gun, Busan 619-902 (KR) (For All Designated States Except US).
BEXCORE INC. [KR/KR]; 6F, 2nd Bldg. of Asan Institute for Life Sciences, Asan Medical Center, 388-1, Pungnap-dong, Songpa-gu, Seoul 138-736 (KR) (For All Designated States Except US).
LEE, Sang Jun [KR/KR]; (KR) (For US Only).
KIM, Young Ok [KR/KR]; (KR) (For US Only).
NAM, Bo-Hye [KR/KR]; (KR) (For US Only)
Inventors: LEE, Sang Jun; (KR).
KIM, Young Ok; (KR).
NAM, Bo-Hye; (KR)
Agent: LEE, Won-Hee; 8th Fl., Sung-Ji Heights II, 642-16 Yoksam-dong, Kangnam-ku, Seoul 135-080 (KR)
Priority Data:
10-2006-0009418 31.01.2006 KR
10-2006-0022389 09.03.2006 KR
Title (EN) PRODUCTION OF A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN BY THE SIGNAL SEQUENCE AND SECRETIONAL ENHANCER
(FR) PROCÉDÉ DE PRODUCTION D'UNE FORME NATIVE SOLUBLE DE PROTÉINE RECOMBINÉE UTILISANT LA SÉQUENCE SIGNAL ET UN AGENT RENFORÇANT LA SÉCRÉTION
Abstract: front page image
(EN)The present invention is drawn to a method for enhancing secretional efficiency of a heterologous protein using a secretional enhancer consisting of a modified signal sequence which comprises the N-region of a signal sequence and/or a hydrophobic fragment of the said signal sequence comprising the said N-region and/or the hydrophilic polypeptide. The method of the present invention can be used not only for production of recombinant heterologous proteins by inhibiting insoluble precipitation and enhancing secretional efficiency of the recombinant protein into the periplasm or the extracellular fluid and but also for transduction of therapeutic proteins by enhancing membrane-permeability of the recombinant protein using a strong secretional enhancer.
(FR)L'invention concerne un procédé permettant de renforcer l'efficacité de sécrétion d'une protéine hétérologue au moyen d'un agent renforçant la sécrétion, ce dernier étant constitué d'une séquence signal modifiée comprenant la région N d'une séquence signal et/ou d'un fragment hydrophobe de ladite séquence signal comprenant ladite région N et/ou d'un polypeptide hydrophile. Le procédé de l'invention est utile non seulement pour produire des protéines hétérologues recombinées par l'inhibition d'un précipité insoluble et le renforcement de l'efficacité de sécrétion de la protéine recombinée dans le périplasme ou le fluide extracellulaire, mais aussi pour la transduction de protéines thérapeutiques par l'accroissement de la perméabilité membranaire de la protéine recombinée à l'aide d'un puissant agent renforçant la sécrétion.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: Korean (KO)