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1. WO2007035929 - METHOD FOR DETECTING AND QUANTITATING MULTIPLE SUBCELLULAR COMPONENTS

Publication Number WO/2007/035929
Publication Date 29.03.2007
International Application No. PCT/US2006/037301
International Filing Date 22.09.2006
Chapter 2 Demand Filed 25.05.2007
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6816
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
C12Q 1/6841
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6841In situ hybridisation
G01N 2333/805
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
795Porphyrin- or corrin-ring-containing peptides
805Haemoglobins; Myoglobins
G01N 2800/385
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2800Detection or diagnosis of diseases
38Pediatrics
385Congenital anomalies
G01N 33/56966
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56966Animal cells
G01N 33/689
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
689related to pregnancy or the gonads
Applicants
  • IKONISYS, INC. [US]/[US] (AllExceptUS)
  • KILPATRICK, Michael [GB]/[US] (UsOnly)
  • TAFAS, Triantafyllos, P. [GR]/[US] (UsOnly)
Inventors
  • KILPATRICK, Michael
  • TAFAS, Triantafyllos, P.
Agents
  • MOORE, Steven, J.
Priority Data
11/233,20022.09.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR DETECTING AND QUANTITATING MULTIPLE SUBCELLULAR COMPONENTS
(FR) PROCEDE PERMETTANT DE DETECTER ET DE QUANTIFIER DE MULTIPLES COMPOSANTS SUBCELLULAIRES
Abstract
(EN)
A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins.
(FR)
La présente invention se rapporte à un procédé permettant de détecter et de quantifier des signaux fluorescents multiples et uniques à partir d'un échantillon cellulaire. Le procédé combine des techniques d'immunohistochimie et d'hybridation in situ à marquage fluorescent. Le procédé selon l'invention est utile pour identifier des composants subcellulaires spécifiques de cellules telles que des chromosomes et des protéines.
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