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1. WO2007035750 - METHOD FOR ISOLATING NUCLEIC ACIDS

Publication Number WO/2007/035750
Publication Date 29.03.2007
International Application No. PCT/US2006/036512
International Filing Date 19.09.2006
IPC
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
C12N 15/1013
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
1013by using magnetic beads
Applicants
  • AGENCOURT BIOSCIENCE CORPORATION [US]/[US] (AllExceptUS)
  • MCKERNAN, Kevin [US]/[US] (UsOnly)
  • ZIAUDDIN, Junaid [BD]/[US] (UsOnly)
Inventors
  • MCKERNAN, Kevin
  • ZIAUDDIN, Junaid
Agents
  • COLLINS, Anne, J.
Priority Data
11/231,36320.09.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR ISOLATING NUCLEIC ACIDS
(FR) PROCÉDÉ POUR L'ISOLEMENT D'ACIDES NUCLÉIQUES
Abstract
(EN)
Described herein is a method in which genomic nucleic acid of a cell can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid (e.g., plasmid DNA) of the cell directly from a cell growth culture. Also described herein, a method in which genomic nucleic acid can be separated from nucleic acid having a molecular weight that is lower than the molecular weight of the genomic nucleic acid in a cell lysate without the need to prepare a cleared lysate. The present invention is also directed to a method of isolating genomic nucleic acid (e.g., RNA, DNA) of a cell or an organism.
(FR)
La présente invention a trait à un procédé dans lequel l'acide nucléique génomique d'une cellule peut être séparé de l'acide nucléique ayant un poids moléculaire qui est inférieur au poids moléculaire de l'acide nucléique génomique (par exemple, l'ADN plasmidique) de la cellule directement à partir d'une culture cellulaire. L'invention a également trait à un procédé dans lequel l'acide nucléique génomique peut être séparé de l'acide nucléique ayant un poids moléculaire qui est inférieur au poids moléculaire de l'acide nucléique génomique dans un lysat cellulaire sans nécessiter la préparation d'un lysat clarifié. La présente invention a trait en outre à un procédé d'isolement de l'acide nucléique génomique (par exemple, l'ARN, l'ADN) d'une cellule ou d'un organisme.
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