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1. WO2007032789 - SYSTEM FOR GEL ELECTROPHORETIC IMMUNOASSAY

Publication Number WO/2007/032789
Publication Date 22.03.2007
International Application No. PCT/US2006/019464
International Filing Date 18.05.2006
IPC
C12M 1/34 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
G01N 33/558 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
558using diffusion or migration of antigen or antibody
CPC
B82Y 10/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
10Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
B82Y 15/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
15Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
B82Y 30/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
30Nanotechnology for materials or surface science, e.g. nanocomposites
G01N 27/447
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
G01N 27/44747
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
416Systems
447using electrophoresis
44704Details; Accessories
44747Composition of gel or of carrier mixture
Applicants
  • SANDIA NATIONAL LABORATORIES [US]/[US] (AllExceptUS)
  • HERR, Amy, Elizabeth [US]/[US]
  • SINGH, Anup, K. [US]/[US]
  • THROCKMORTON, Daniel, J. [US]/[US]
Inventors
  • HERR, Amy, Elizabeth
  • SINGH, Anup, K.
  • THROCKMORTON, Daniel, J.
Agents
  • EVANS, Timothy, P.
Priority Data
11/137,74424.05.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SYSTEM FOR GEL ELECTROPHORETIC IMMUNOASSAY
(FR) SYSTEME POUR IMMUNODOSAGE ELECTROPHORETIQUE SUR GEL
Abstract
(EN)
A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes, native PAGE conditions were employed. Both direct (non-competitive) and competitive immunoassay formats are demonstrated in microchips for detecting toxins and biomarkers (cytokines, c-reactive protein) in bodily fluids (serum, saliva, oral fluids). Further, a description of gradient gels fabrication is included, in an effort to describe methods we have developed for further optimization of on-chip PAGE immunoassays. The described chip-based PAGE immunoassay method enables immunoassays are fast (minutes) and require very small amounts of sample (less than a few microliters). Use of microfabricated chips as a platfo&pgr;n enables integration, parallel assays, automation and development of portable devices.
(FR)
Une plate-forme micro-analytique de réalisation d'immunodosages basés sur une électrophorèse a été développée par intégration de gels de polyacrylamide réticulés photopolymérisés au sein d'un dispositif microfluidique. Ces immunodosages microfluidiques sont obtenus par séparation électrophorétique sur gel et quantification d'une concentration d'analytes basée sur une électrophorèse traditionnelle sur gel de polyacrylamide (PAGE). Pour retenir une activité biologique de protéines et maintenir des complexes immuns intacts, des conditions PAGE natives sont utilisées. Des formats d'immunodosages directs (non compétitifs) et compétitifs sont démontrés dans des micropuces de manière à détecter des toxines et des biomarqueurs (cytokines, protéine c-réactive) dans des fluides corporels (sérum, salive, liquides buccaux). En outre, cette invention a aussi pour objet une description de la fabrication de gels de gradient dans un souci de décrire des méthodes développées pour l'optimisation d'immunodosages PAGE sur puce. Cette méthode d'immunodosage PAGE basée sur une puce permet d'activer des immunodosages qui sont rapides (agissant en quelques minutes) et nécessitent de très petites quantités d'échantillon (moins de quelques microlitres). L'utilisation de puces microfabriquées en tant que plate-forme permet une intégration, des dosages parallèles, l'automatisation et le développement de dispositifs portables.
Also published as
EP6824755
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