Processing

Please wait...

Settings

Settings

Goto Application

1. WO2007028983 - PROTEIN ASSAY

Publication Number WO/2007/028983
Publication Date 15.03.2007
International Application No. PCT/GB2006/003293
International Filing Date 06.09.2006
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
C07K 14/76 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
76Albumins
CPC
G01N 2333/76
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
435from animals; from humans
76Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
G01N 33/68
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
G01N 33/6815
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6806Determination of free amino acids
6812Assays for specific amino acids
6815containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
G01N 33/6842
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
Applicants
  • THE NOTTINGHAM TRENT UNIVERSITY [GB]/[GB] (AllExceptUS)
  • CHAHINE, Samir [LB]/[GB] (UsOnly)
  • LIVINGSTONE, Callum [GB]/[GB] (UsOnly)
  • DAVIS, James [GB]/[GB] (UsOnly)
Inventors
  • CHAHINE, Samir
  • LIVINGSTONE, Callum
  • DAVIS, James
Agents
  • ELSY, David
Priority Data
0518460.109.09.2005GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROTEIN ASSAY
(FR) DOSAGE DE PROTÉINES
Abstract
(EN)
The invention provides a method for determining the relative amount of a sulphydryl-containing protein, such as albumin, compared with lower molecular weight thiol-containing compounds in a sample containing both sulphydryl containing protein and lower molecular weight thiol-containing compounds, comprising the step of contacting the sample with substituted or non-substituted 2.2'-dithio(bis)benzothiazole reagent (the first reagent) or a derivative thereof and determining directly or indirectly an amount of reacted thiol compounds that have reacted with the first reagent (reacted thiols) and/or thiol-containing compounds that have not reacted (unreacted thiol) with the first reagent. The method allows the discrimination between, for example, albumin and low molecular weight species such as glutathione, cysteine and homocysteine. The first reagent may be provided in a column and the sample passed through the column.
(FR)
La présente invention concerne un procédé de détermination de la quantité relative d'une protéine contenant un sulfhydryle, telle que l'albumine, par rapport à la quantité de composés contenant un thiol à masse moléculaire plus faible dans un échantillon qui contient à la fois une protéine contenant un sulfhydryle et des composés contenant un thiol à masse moléculaire plus faible. Le procédé comprend une étape de mise en contact de l'échantillon avec un réactif 2.2'-dithio(bis) benzothiazole substitué ou non substitué (premier réactif) ou avec un de ses dérivés et la détermination directe ou indirecte de la quantité de composés contenant un thiol qui ont réagi avec le premier réactif (thiols ayant réagi) et/ou de la quantité de composés contenant un thiol qui n'ont pas réagi avec le premier réactif (thiols n'ayant pas réagi). Le procédé permet par exemple de différencier l'albumine d'espèces à masse moléculaire faible telles que le glutathion, la cystéine ou l'homocystéine. Le premier réactif peut être placé dans une colonne à travers laquelle on fait passer l'échantillon.
Also published as
EP06779312
EP6779312
Latest bibliographic data on file with the International Bureau