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Pub. No.:    WO/2007/022281    International Application No.:    PCT/US2006/032001
Publication Date: 22.02.2007 International Filing Date: 16.08.2006
C02F 1/40 (2006.01), G01N 27/00 (2006.01), G01N 33/558 (2006.01)
Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY [US/US]; 77 Massachusetts Ave., Cambridge, MA 02139 (US) (For All Designated States Except US).
HAN, Jongyoon [KR/US]; (US) (For US Only).
SONG, Yong-Ak [KR/US]; (US) (For US Only)
Inventors: HAN, Jongyoon; (US).
SONG, Yong-Ak; (US)
Agent: COHEN, Mark, S.; Pearl Cohen Zedek Latzer, LLP, 1500 Broadway, 12th Floor, New York, NY 10036 (US)
Priority Data:
60/708,374 16.08.2005 US
Abstract: front page image
(EN)This invention is directed to methods and devices for separating molecules in a sample, based on differences in their isoelectric point (pI). The methods and devices make use of a diffusion potential created in a microfluidic chamber when a buffered solution comprising molecules, which differ in terms of their isoelectric point (pI) values and a second buffer, which differs from the buffered solution in terms of its pH or salt concentration are introduced in the chamber. The diffusion potential, in turn, enables charge-based separation of the molecules. Applications and permutations of the methods and devices are described.
(FR)L’invention concerne des procédés et dispositifs de séparation de molécules dans un échantillon, basés sur les différences de leur point isoélectrique (pi). Lesdits procédés et dispositifs emploient un potentiel de diffusion créé dans une chambre microfluide lorsqu’une solution tamponnée comprenant des molécules, qui diffèrent en termes de valeurs de leur point isoélectrique (pi) et une deuxième solution tampon, qui diffère de la solution tamponnée en termes de pH ou de concentration de sel, sont introduites dans la chambre. Le potentiel de diffusion rend à son tour possible la séparation des molécules en fonction de leur charge. L’invention concerne également des applications et permutations desdits procédés et dispositifs.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)