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1. (WO2007011902) METHODS FOR RNA PROFILING
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/011902 International Application No.: PCT/US2006/027754
Publication Date: 25.01.2007 International Filing Date: 17.07.2006
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
APPLERA CORPORATION [US/US]; 850 Lincoln Centre Drive Foster City, CA 94404, US (AllExceptUS)
LAO, Kai Qin [US/US]; US (UsOnly)
LIVAK, Kenneth [US/US]; US (UsOnly)
Inventors:
LAO, Kai Qin; US
LIVAK, Kenneth; US
Agent:
FINN, Andrew ; APPLERA CORPORATION 850 Lincoln Centre Drive Foster City, CA 94404, US
Priority Data:
60/699,95315.07.2005US
60/710,38023.08.2005US
Title (EN) METHODS FOR RNA PROFILING
(FR) PROCEDES DE PROFILAGE D'ARN
Abstract:
(EN) The present teachings provide methods, compositions, and kits for detecting micro RNAs (miRNAs). In some embodiments, the miRNAs are quantified from formalin-fixed paraffin-embedded tissue samples in which messenger RNA is degraded. The present teachings take advantage of the observation that most mature miRNAs in vivo are protected by degradation as a result of their association with RISC. Thus, novel methods of studying nucleic acids in archived tissues containing degraded messenger RNA are provided, wherein RISC-protected miRNAs are liberated, and analyzed.
(FR) La présente invention concerne de procédés, des compositions et des kits permettant de détecter des micros ARN (ARMmi). Dans certains modes de réalisation de l'invention, ces ARNmi sont quantifiés à partir d'échantillons de tissus intégrés dans la paraffine fixés au formol, dans lesquels un ARN messager est dégradé. Cette invention tire profit des observations que des ARNmi plus matures in vivo sont protégés par la dégradation du fait de leur association avec RISC. Ainsi, ces nouveaux procédés d'études d'acides nucléiques dans des tissus archivés contenant un ARN messager dégradé sont présentés, des ARNmi protégés par RISC étant libérés, et analysés.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1917367JP2009501531AU2006270063