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1. (WO2007011901) HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2007/011901 International Application No.: PCT/US2006/027753
Publication Date: 25.01.2007 International Filing Date: 17.07.2006
IPC:
C07H 21/04 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
Applicants:
LAO, Kai Qin [US/US]; US (UsOnly)
STRAUS, Neil [US/US]; US (UsOnly)
LIVAK, Kenneth [US/US]; US (UsOnly)
APPLERA CORPORATION [US/US]; 850 Lincoln Centre Drive Foster City, CA 94404, US (AllExceptUS)
Inventors:
LAO, Kai Qin; US
STRAUS, Neil; US
LIVAK, Kenneth; US
Agent:
FINN, Andrew ; Applera Corporation 850 Lincoln Centre Drive Foster City, CA 94404, US
Priority Data:
60/699,96715.07.2005US
Title (EN) HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN
(FR) TRANSCRIPTION INVERSE A AMORÇAGE CHAUD PAR UNE CONCEPTION D'AMORCE
Abstract:
(EN) The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.
(FR) La présente invention concerne des procédés, des compositions et des kits permettant d'effectuer des réactions d'extension d'amorce. Dans certains modes de réalisation de l'invention, une réaction de transcription inverse est effectuée sur un polynucléotide cible avec une amorce d'amorçage à chaud comprenant une souche auto complémentaire à extrémité émoussée et une boucle, et des produits d'extension formés à haute température mais une formation de produits d'extension réduite à faible température.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)