Processing

Please wait...

Settings

Settings

1. WO2007011595 - NEURAL REGENERATION PEPTIDES AND ANTIOXIDANTS PROTECT NEURONS FROM DEGENERATION

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

We claim:

1. A composition for promoting neuroprotection in a mammal having a disease or condition likely to result in neurodegeneration or neural cell death, comprising:
a neural regeneration peptide (NRP); and
an antioxidant (AO).

2. The composition of claim 1, wherein said NRP is selected from the group consisting of peptides having the sequence of SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO: 14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28 and SEQ ID NO:29.

3. The composition of claim 1, wherein said antioxidant is selected from the group consisting of but no limited to vitamin A (retinol), vitamin C (ascorbic acid) and vitamin E (tocotrienol or tocopherol), coenzyme QlO (idebenone), catalase, thioredoxins, thioredoxin reductase 1, peroxiredoxin 2, peroxiredoxin 4, peroxiredoxin 6, Cu/Zn superoxide dismutase, Mn superoxide dismutase, glutathione, L-carnosine, lycopene, lutein, alpha-carotene, beta-carotene, zeaxanthin, astaxanthin, resveratrol, kaempferol, myricetin, isorhamnetin, proanthocyanidins, quercetin, rutin, luteolin, apigenin, tangeritin, hesperetin, naringenin, eriodictyol, catechin, gallocatechin, epicatechin, epigallocatechin, theaflavins, thearubigin, genistein, daidzein, glycitein; rosmarinic acid, cinnamic acid, chlorogenic acid, chicoric, gallotannins, ellagitannins, curcumin, acetylcysteine, lipoic acid (α-lipoic acid) and methionine.

4. A method for protecting neurons from degeneration or death, comprising:
administering to a subject having a condition in which neurons would be destined to degenerate or die, a composition comprising a neural regeneration peptide (NRP) and an antioxidant (AO).

5. The method of claim 4, wherein said NRP is selected from the group consisting of peptides having the sequence of SEQ ID NO:1, SEQ E) NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO: 12, SEQ ID NO:13, SEQ ID NO: 14, SEQ ID NO:15, SEQ DD NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ED NO: 19, SEQ ID NO:20, SEQ ID N0:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ED NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ DD NO:28 and SEQ DD NO:29.

6. The method of claim 4, wherein said AO is selected from the group consisting of but not limited to vitamin A (retinol), vitamin C (ascorbic acid) and vitamin E (tocotrienol or tocopherol), coenzyme QlO (idebenone), catalase, thioredoxins, thioredoxin reductase 1, peroxiredoxin 2, peroxiredoxin 4, peroxiredoxin 6, Cu/Zn superoxide dismutase, Mn superoxide dismutase, glutathione, L-carnosine, lycopene, lutein, alpha-carotene, beta-carotene, zeaxanthin, astaxanthin, resveratrol, kaempferol, myricetin, isorhamnetin, proanthocyanidins, quercetin, rutin, luteolin, apigenin, tangeritin, hesperetin, naringenin, eriodictyol, catechin, gallocatechin, epicatechin, epigallocatechin, theaflavins, thearubigin, genistein, daidzein, glycitein; rosmarinic acid, cinnamic acid, chlorogenic acid, chicoric, gallotannins, ellagitannins, curcumin, acetylcysteine, lipoic acid (α-lipoic acid) and methionine.

7. The method of any of claims 4-6, wherein said disease or condition is selected from the group consisting of bacterial, viral, spirochetal or parasitic infections of the central nervous system, pyrogenic infections, acute bacterial meningitis, leptomeningitis, stroke, ischemic stroke, atherosclerotic thrombosis, lacunes, embolism, hypertensive haemorrhage, ruptured aneurysms, vascular malformations, transient ischemic attacks, intracranial haemorrhage, spontaneous subarachnoid haemorrhage, hypertensive encephalopathy, inflammatory diseases of the brain arteries, decreased perfusion caused by, for example, cardiac insufficiency craniocerebral trauma, basal skull fractures, cranial nerve injuries, carotid-cavernous fistula, pneumocephalus, aerocele andrhinorrhea, cerebral contusion, traumatic intracerebral haemorrhage, acute brain swelling, neuromyelitis optica, acute disseminated encephalomyelitis, acute and subacute necrotizing haemorrhagic encephalitis, diffuse cerebral sclerosis of Schilder, multiple sclerosis in conjunction with peripheral neuropathy, dementia, diffuse cerebral atrophy, diffuse cortical atrophy of the non-Alzheimer type, Lewy body dementia, Pick's disease, fronto-temporal dementia, thalamic degeneration, non-Huntingtonian types of Chorea and dementia, cortico-spinal degeneration (Jakob), the dementia-Parkinson-amyotrophic lateral sclerosis complex, guamanina, peripheral neuropathy, autoimmune disorders, nutritional deficiencies, alcoholism, vascular and metabolic disorders, diabetic neuropathy, confusion, stupor, coma-ischemia-hypoxia, hypoglycaemia, hyperglycemia, hypercapnia, hepatic failure and Reye syndrome, metabolic diseases presenting as a progressive extrapyramidal syndrome, ataxia, hyperthermia, celiac-sprue disease, psychosis, Cushing disease and steroi-nduced encephalopathy, thyroid psychosis and hypothyroidism and pancreatic encephalopathy, nervous system due to nutritional deficiency, alcohol, alcoholism, opiates and synthetic analgesics, sedative hypnotic drugs, stimulants, psychoactive drugs, bacterial toxins, plant poisons, venomous bites and stings, heavy metals, industrial toxins, anti-neoplastic and immunosuppressive agents, thalidomide, aminoglycoside antibiotics (ototoxicity) and penicillin derivatives, cardioprotective agents, beta-blockers, digitalis derivatives and amiodarone.

8. A method for protecting neurons in cell culture, comprising:
preparing and culturing neuronal cells in the presence of a neural regeneration peptide (NRP) and/or an antioxidant (AO).

9. The method of claim 8, wherein said NRP is selected from the group consisting of peptides having the sequence of SEQ TD NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO:13, SEQ ID NO: 14, SEQ ID NO:15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28 and SEQ ID NO:29.

10. The method of claim 8, wherein said antioxidant is selected from the group consisting of but no limited to vitamin A (retinol), vitamin C (ascorbic acid) and vitamin E (tocotrienol or tocopherol), coenzyme QlO (idebenone), catalase, thioredoxins, thioredoxin reductase 1, peroxiredoxin 2, peroxiredoxin 4, peroxiredoxin 6, Cu/Zn superoxide dismutase, Mn superoxide dismutase, glutathione, L-carnosine, lycopene, lutein, alpha-carotene, beta-carotene, zeaxanthin, astaxanthin, resveratrol, kaempferol, myricetin, isorhamnetin, proanthocyanidins, quercetin, rutin, luteolin, apigenin, tangeritin, hesperetin, naringenin, eriodictyol, catechin, gallocatechin, epicatechin, epigallocatechin, theaflavins, thearubigin, genistein, daidzein, glycitein; rosmarinic acid, cinnamic acid, chlorogenic acid, chicoric, gallotannins, ellagitannins, curcumin, acetylcysteine, lipoic acid (a-lipoic acid) and methionine.

11. A method for protecting neurons in cell culture, comprising:
dissociating neuronal cells in a cell culture medium containing an antioxidant; and
culturing said cells in a cell culture medium containing an NRP.

12. A kit for culturing neuronal cells, comprising:
at least one NRP;
at least one AO;
a mixing vial;
a solvent for dissolving said NRP and said AO;
and instructions for use.

13. The kit of claim 12, further comprising a cell culture medium.