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1. (WO2007010620) CELL TRANSFECTION ARRAY FOR USE IN TRANSFECTION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/010620 International Application No.: PCT/JP2005/013500
Publication Date: 25.01.2007 International Filing Date: 22.07.2005
IPC:
C12N 15/87 (2006.01) ,C12M 1/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
Applicants:
株式会社高研 KOKEN CO., LTD. [JP/JP]; 〒1710031 東京都豊島区目白3丁目14番3号 Tokyo 14-3, Mejiro 3-chome, Toshima-ku, Tokyo 1710031, JP (AllExceptUS)
大日本住友製薬株式会社 DAINIPPON SUMITOMO PHARMA CO., LTD. [JP/JP]; 〒5418524 大阪府大阪市中央区道修町2丁目6番8号 Osaka 6-8, Dosho-machi 2-chome, Chuo-ku, Osaka-shi, Osaka 5418524, JP (AllExceptUS)
本間 紀美 HONMA, Kimi [JP/JP]; JP (UsOnly)
落谷 孝広 OCHIYA, Takahiro [JP/JP]; JP (UsOnly)
Inventors:
本間 紀美 HONMA, Kimi; JP
落谷 孝広 OCHIYA, Takahiro; JP
Agent:
庄司 隆 SHOJI, Takashi; 〒1010032 東京都千代田区岩本町3丁目2番10号 SN岩本町ビル6階 Tokyo 6F, SN Iwamotocho Bldg. 2-10, Iwamotocho 3-chome Chiyoda-ku, Tokyo 1010032, JP
Priority Data:
Title (EN) CELL TRANSFECTION ARRAY FOR USE IN TRANSFECTION
(FR) MATRICE DE TRANSFECTION CELLULAIRE POUR UTILISATION DANS LA TRANSFECTION
(JA) 核酸導入用セルトランスフェクションアレイ
Abstract:
(EN) Disclosed is a transfection microarray which enables the introduction of a nucleic acid into a cell and the expression of the nucleic acid only by adding the nucleic acid to a plate or the like and then seeding and culturing the cell therein without the need of addition of a transfection reagent or an additive. A transfection microarray comprising atelo-collagen, a transfecting gent and a nucleic acid in a plate or the like. A cell into which the nucleic acid is to be introduced is seeded to the microarray and then cultured to thereby introduce the nucleic acid into the cell without the need of preparation of a viral vector or a mixture of a nucleic acid with a transfecting agent or without the need of addition of a transfecting agent or an additive after the cell is cultured.
(FR) L’invention a pour objet un microréseau de transfection qui permet l'introduction d'un acide nucléique dans une cellule et l'expression de l'acide nucléique en ajoutant simplement l'acide nucléique à une plaque ou similaire, et ensuite en y ensemençant et en y cultivant la cellule sans qu’il soit nécessaire d'ajouter un réactif de transfection ou un additif. Il s’agit d’un microréseau de transfection qui comprend de l’Atelocollagène, un agent transfectant et un acide nucléique dans une plaque ou similaire. Une cellule, dans laquelle l'acide nucléique sera introduit, est ensemencée sur le microréseau et ensuite cultivée pour ainsi introduire l'acide nucléique dans la cellule sans qu’il soit nécessaire de préparer un vecteur viral ou un mélange d'un acide nucléique avec un agent transfectant, ni d'ajouter un agent transfectant ou un additif après que la cellule est cultivée.
(JA)  本発明は、プレート等に核酸を添加したのちに核酸導入試薬や添加剤を添加することなく、そこに細胞を播種、培養するだけで該細胞に核酸を導入し、発現させることができる核酸導入用マイクロアレイを提供することを課題とする。  アテロコラーゲン、遺伝子導入剤および核酸をプレート等に備えた核酸導入用マイクロアレイを調製することによる。該マイクロアレイに核酸導入用細胞を播種し、培養することで、細胞培養後にウイルスベクターや核酸と核酸導入剤の混合物を調製する必要なく、または核酸導入剤や添加剤の添加の必要なく核酸を細胞に導入することができる。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
EP1921153US20090093058