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1. (WO2007010293) MEMBRANE-TRANSLOCATING PEPTIDES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/010293 International Application No.: PCT/GB2006/002766
Publication Date: 25.01.2007 International Filing Date: 24.07.2006
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
ISOGENICA LTD [GB/GB]; Babraham Hall Babraham Cambridgeshire CB2 4AT, GB (AllExceptUS)
COOMBER, David [AU/AU]; AU (UsOnly)
FITZGERALD, Kevin [GB/GB]; GB (UsOnly)
MCGREGOR, Duncan [GB/GB]; GB (UsOnly)
ULLMAN, Chris [GB/GB]; GB (UsOnly)
LEANDERSON, Tomas [SE/SE]; SE (UsOnly)
Inventors:
COOMBER, David; AU
FITZGERALD, Kevin; GB
MCGREGOR, Duncan; GB
ULLMAN, Chris; GB
LEANDERSON, Tomas; SE
Agent:
CALDWELL, Judith, Margaret ; Keltie Fleet Place House 2 Fleet Place London EC4M 7ET, GB
Priority Data:
0515115.422.07.2005GB
Title (EN) MEMBRANE-TRANSLOCATING PEPTIDES
(FR) PEPTIDES CAPABLES D'UNE TRANSLOCATION DANS UNE MEMBRANE
Abstract:
(EN) A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.
(FR) L'invention concerne un procédé servant à sélectionner des peptides capables d'une translocation dans une membrane (MTP) à partir d'une banque de peptides exprimés qui sont capables de traverser ou de pénétrer dans une membrane lipidique. On exprime une pluralité de constructions d'acides nucléiques qui codent pour des peptides exprimés, ce qui entraîne la formation d'une pluralité de complexes acide nucléique-peptide, chaque complexe comprenant au moins un peptide exprimé associé à la construction d'acides nucléiques correspondante codant pour le peptide exprimé ; on expose les complexes à un ensemble de compartiments encapsulés dans une membrane, permettant ainsi à une réaction de translocation d'avoir lieu ; on enlève les complexes qui restent non associés avec la membrane ; facultativement on enlève les complexes qui sont associés à la membrane ; et on récupère les complexes acide nucléique-peptide internalisés. Les compartiments encapsulés dans une membrane peuvent être des vésicules artificielles telles que des liposomes ou des ensembles d'un ou plusieurs types de cellules.
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Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1907544JP2009502132ES2360420US20080287311CN101258242CA2658491
PT1907544AU2006271395DK1907544IN159/DELNP/2008