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1. (WO2007010254) METHODS OF NUCLEIC ACID AMPLIFICATION AND SEQUENCING
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/010254 International Application No.: PCT/GB2006/002693
Publication Date: 25.01.2007 International Filing Date: 20.07.2006
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
SOLEXA LIMITED [GB/GB]; Chesterford Research Park Little Chesterford Saffron Walden Essex CB10 1XL, GB (AllExceptUS)
BOUTELL, Jonathan, Mark [GB/GB]; GB (UsOnly)
SMITH, Geoffrey, Paul [GB/GB]; GB (UsOnly)
COX, Anthony, James [GB/GB]; GB (UsOnly)
EARNSHAW, David, James [GB/GB]; GB (UsOnly)
SCHROTH, Gary, Paul [US/GB]; GB (UsOnly)
Inventors:
BOUTELL, Jonathan, Mark; GB
SMITH, Geoffrey, Paul; GB
COX, Anthony, James; GB
EARNSHAW, David, James; GB
SCHROTH, Gary, Paul; GB
Agent:
WHITE, Nina, Louise ; Boult Wade Tennant Verulam Gardens 70 Gray's Inn Road London WC1X 8BT, US
Priority Data:
0514909.120.07.2005GB
Title (EN) METHODS OF NUCLEIC ACID AMPLIFICATION AND SEQUENCING
(FR) MÉTHODES D'AMPLIFICATION ET DE SÉQUENCAGE D’ACIDES NUCLÉIQUES
Abstract:
(EN) The invention relates to a method of amplifying one or more nucleic acid templates on a solid support in a nucleic acid amplification reaction, for example by solid-phase PCR using one or more amplification primers attached to the solid support. The method is characterised in that the amplification primers used comprise a template-specific portion which is a sequence of at least 26 consecutive nucleotides and are not capable of annealing to target regions in the template under conditions of the amplification reaction. The method is particularly useful for amplifying human genomic DNA.
(FR) L'invention concerne une méthode pour amplifier une ou plusieurs matrices d’acides nucléiques sur un support solide dans une réaction d'amplification d’acides nucléiques, par exemple par PCR en phase solide en utilisant une ou plusieurs amorces d'amplification attachées au support solide. La méthode est caractérisée en ce que les amorces d'amplification utilisées comprennent une portion spécifique à la matrice qui est une séquence d'au moins 26 nucléotides consécutifs et qui n'est pas capable d'annelage à des régions cibles dans la matrice dans des conditions de réaction d'amplification. La méthode est particulièrement utile pour amplifier l’ADN génomique humain.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1910561US20090117621