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1. (WO2007010251) PREPARATION OF TEMPLATES FOR NUCLEIC ACID SEQUENCING
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/010251 International Application No.: PCT/GB2006/002687
Publication Date: 25.01.2007 International Filing Date: 20.07.2006
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
SOLEXA LIMITED [GB/GB]; Chesterford Research Park Little Chesterford Saffron Walden Essex CB10 1XL, GB (AllExceptUS)
LIU, Xiaohai [CN/GB]; GB (UsOnly)
MILTON, John [GB/GB]; GB (UsOnly)
SMITH, Geoffrey Paul [GB/GB]; GB (UsOnly)
BARNES, Colin [GB/GB]; GB (UsOnly)
RASOLONJATOVO, Isabelle Marie Julia [FR/GB]; GB (UsOnly)
RIGATTI, Roberto [IT/GB]; GB (UsOnly)
WU, Xiaolin [CN/GB]; GB (UsOnly)
OST, Tobias William Barr [GB/GB]; GB (UsOnly)
WORSLEY, Graham John [GB/GB]; GB (UsOnly)
EARNSHAW, David James [GB/GB]; GB (UsOnly)
TURCATTI, Gerardo [CH/CH]; CH (UsOnly)
ROMIEU, Anthony [FR/FR]; FR (UsOnly)
Inventors:
LIU, Xiaohai; GB
MILTON, John; GB
SMITH, Geoffrey Paul; GB
BARNES, Colin; GB
RASOLONJATOVO, Isabelle Marie Julia; GB
RIGATTI, Roberto; GB
WU, Xiaolin; GB
OST, Tobias William Barr; GB
WORSLEY, Graham John; GB
EARNSHAW, David James; GB
TURCATTI, Gerardo; CH
ROMIEU, Anthony; FR
Agent:
WHITE, Nina Louise ; BOULT WADE TENNANT Verulam Gardens 70 Gray's Inn Road London WC1X 8BT, GB
Priority Data:
0514936.420.07.2005GB
Title (EN) PREPARATION OF TEMPLATES FOR NUCLEIC ACID SEQUENCING
(FR) PREPARATION DE MATRICES POUR SEQUENCAGE D'ACIDES NUCLEIQUES
Abstract:
(EN) The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
(FR) Cette invention concerne des procédés de génération de matrices pour une réaction de séquençage d'acides nucléiques qui consistent: à utiliser au moins une molécule d'acide nucléique double brin, les deux brins de la molécule d'acide nucléique double brin étant fixés à un support solide au niveau de l'extrémité 5', à réaliser le clivage de l'un ou des deux brins de la molécule d'acide nucléique double brin puis à soumettre le ou les brins clivés à des conditions de dénaturation pour éliminer la partie du ou des brins clivés non fixée au support solide, ce qui génère une matrice partiellement ou substantiellement simple brin pour une réaction de séquençage d'acides nucléiques.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP1910559EP2298930US20090118128