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1. (WO2007008722) METHOD FOR THE ISOLATION OF RNA FROM BIOLOGICAL SOURCES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2007/008722    International Application No.:    PCT/US2006/026618
Publication Date: 18.01.2007 International Filing Date: 07.07.2006
IPC:
C07H 21/00 (2006.01)
Applicants: SIGMA-ALDRICH CO. [US/US]; 3050 Spruce Street, St. Louis, Missouri 63103 (US) (For All Designated States Except US).
CHEN, Fuqiang [US/US]; (US) (For US Only).
CUTTER, David [US/US]; (US) (For US Only)
Inventors: CHEN, Fuqiang; (US).
CUTTER, David; (US)
Agent: MCBRIDE, Timothy, B.; Senniger Powers, #1 Metropolitan Square, 16th Floor, St. Louis, Missouri 63102 (US)
Priority Data:
11/180,087 13.07.2005 US
Title (EN) METHOD FOR THE ISOLATION OF RNA FROM BIOLOGICAL SOURCES
(FR) PROCEDE PERMETTANT D'ISOLER L'ARN DE SOURCES BIOLOGIQUES
Abstract: front page image
(EN)Methods and kits for isolating RNA are provided that enable rapid RNA preparation from biological sources. In one aspect, RNA is isolated from difficult plant tissues and cells that contain high levels of secondary metabolites, without employing organic extraction or salt precipitation procedures. This method employs novel lysing and binding conditions to allow preparation of RNA free from secondary metabolites.
(FR)L'invention porte sur des procédés et des trousses d'isolement d'ARN qui permettent de préparer rapidement de l'ARN en provenance de sources biologiques. Dans un aspect, on isole l'ARN de cellules et tissus végétaux difficiles contenant des niveaux élevés de métabolites secondaires, sans recourir à des opérations d'extraction organique ou de précipitation de sels. Le procédé de l'invention fait appel à des conditions de lyse et de liaison novatrices qui permettent de préparer un ARN dépourvu de métabolites secondaires.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)