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1. (WO2007007933) METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/007933 International Application No.: PCT/KR2005/003442
Publication Date: 18.01.2007 International Filing Date: 14.10.2005
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY [KR/KR]; 373-1 Guseong-dong, Yuseong-gu Daejeon 305-701, KR (AllExceptUS)
LEE, Sang Yup [KR/KR]; KR (UsOnly)
KIM, Tae Yong [KR/KR]; KR (UsOnly)
LEE, Dong Yup [KR/KR]; KR (UsOnly)
Inventors:
LEE, Sang Yup; KR
KIM, Tae Yong; KR
LEE, Dong Yup; KR
Agent:
LEE, Cheo-Young; 11th Floor, Won Bldg., 648-26, Yeoksam-dong, Gangnam-gu Seoul 135-080, KR
Priority Data:
10-2005-006240411.07.2005KR
Title (EN) METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES
(FR) PROCEDE D'AMELIORATION IN SILICO D'ORGANISMES UTILISANT LA SOMME DES FLUX DE METABOLITES
Abstract:
(EN) The present invention relates to an in silico method for improving an organism on the basis of the flux sum (Φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.
(FR) La présente invention a trait à un procédé in silico pour l'amélioration d'un organisme fondé sur la somme des flux ($g(F)) de métabolites, et plus particulièrement à un procédé pour le criblage de métabolites clés qui accroissent la performance de production d'une substance utile, le procédé comprenant la définition de l'utilisation de métabolites d'un organisme pour produire une substance utile sous la forme d'une somme des flux et la perturbation de la somme des flux, ainsi qu'à un procédé pour l'amélioration d'un organisme produisant une substance utile, le procédé comprenant la délétion et/ou l'amplification de gènes associés aux dits métabolites clés criblés. Selon la présente invention, la corrélation entre des métabolites spécifiques et la production de substance utile peut être prédite de manière précise, de sorte qu'il soit possible de développer un organisme ayant une production de substance utile accrue par l'introduction et/ou l'amplification et/ou la délétion de gènes exprimant des enzymes associées aux métabolites spécifiques. En outre, il est également possible d'accroître la production d'une substance utile par l'ajout de métabolites spécifiques pendant la culture.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: Korean (KO)
Also published as:
EP1915459US20090215048