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1. (WO2007007777) TRANSGENIC NON-HUMAN MAMMAL CAPABLE OF EXPRESSING NON-FLUORESCENT MUTANT OF FLUORESCENT PROTEIN
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/007777 International Application No.: PCT/JP2006/313826
Publication Date: 18.01.2007 International Filing Date: 12.07.2006
Chapter 2 Demand Filed: 30.11.2006
IPC:
C12N 15/00 (2006.01) ,A01K 67/027 (2006.01) ,C07K 14/435 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K
ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67
Rearing or breeding animals, not otherwise provided for; New breeds of animals
027
New breeds of vertebrates
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
国立大学法人京都大学 KYOTO UNIVERSITY [JP/JP]; 〒6068501 京都府京都市左京区吉田本町36番地1 Kyoto 36-1, Yoshida-honmachi, Sakyo-ku, Kyoto-shi, Kyoto 6068501, JP (AllExceptUS)
武藤 誠 TAKETO, Makoto [JP/JP]; JP (UsOnly)
園下 将大 SONOSHITA, Masahiro [JP/JP]; JP (UsOnly)
中根 崇智 NAKANE, Takanori [JP/JP]; JP (UsOnly)
Inventors:
武藤 誠 TAKETO, Makoto; JP
園下 将大 SONOSHITA, Masahiro; JP
中根 崇智 NAKANE, Takanori; JP
Agent:
特許業務法人特許事務所サイクス SIKs & Co.; 〒1040031 東京都中央区京橋一丁目8番7号 京橋日殖ビル8階 Tokyo 8th Floor, Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chome Chuo-ku, Tokyo 1040031, JP
Priority Data:
2005-20246512.07.2005JP
Title (EN) TRANSGENIC NON-HUMAN MAMMAL CAPABLE OF EXPRESSING NON-FLUORESCENT MUTANT OF FLUORESCENT PROTEIN
(FR) MAMMIFÈRE TRANSGÉNIQUE NON HUMAIN CAPABLE D’EXPRIMER UN MUTANT NON FLUORESCENT D’UNE PROTÉINE FLUORESCENTE
(JA) 蛍光性蛋白質の非蛍光性変異体を発現するトランスジェニック非ヒト哺乳動物
Abstract:
(EN) Disclosed is a laboratory animal exerting no immunogenic response against a cultured cancer cell labeled with a fluorescent protein or luciferase, which is useful in cancer cell transplantation experiments, transplantation experiments of stem cells derived from various organs or basic experiments for regenerative therapy. A substantially non-fluorescent GFP mutant which has substitution, deletion or insertion at least at one position selected from position 66, position 67 and position 68 of the amino acid sequence for EGFP (e.g., a protein depicted in SEQ ID NO:2 in Sequence Listing); a transgenic non-human mammal capable of expressing the protein; and others.
(FR) L’invention concerne un animal de laboratoire n’exerçant pas de réponse immunogène à l’encontre d’une cellule cancéreuse cultivée marquée par une protéine fluorescente ou une luciférase, qui est utile pour les expériences de transplantation des cellules cancéreuses, les expériences de transplantation des cellules souches dérivées de divers organes ou les expériences de recherche fondamentale en matière de thérapie régénérative. L’invention a trait à un mutant GFP sensiblement non fluorescent qui possède une substitution, une suppression ou une addition à au moins une position choisie parmi la position 66, la position 67 et la position 68 de la séquence d’acides aminés pour l’EGFP (par exemple, une protéine présentée à la SEQ ID NO:2 dans la liste des séquences) ; un mammifère transgénique non humain capable d’exprimer la protéine ; et autres.
(JA)  癌細胞の移植実験及び多様な臓器の幹細胞移植実験や再生医療の基礎実験に有用な、蛍光性蛋白質やルシフェラーゼにより標識された培養癌細胞に対する免疫反応を回避した実験動物、例えば、EGFPアミノ酸配列の66位、67位、及び68位から選択される一個以上の位置に置換、欠失、又は挿入があり、かつ実質的に非蛍光性であるGFP変異体(例えば、配列表の配列番号2に記載の蛋白質等)及び該蛋白質を発現するトランスジェニック非ヒト哺乳動物を提供する。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)