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1. (WO2007007053) MODIFIED AMYLASE FROM PSEUDOMONAS SACCHAROPHILIA
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/007053 International Application No.: PCT/GB2006/002513
Publication Date: 18.01.2007 International Filing Date: 07.07.2006
Chapter 2 Demand Filed: 07.05.2007
IPC:
C12N 9/28 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
24
acting on glycosyl compounds (3.2)
26
acting on alpha-1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
28
Alpha-amylase from microbial source, e.g. bacterial amylase
Applicants:
DANISCO A/S [DK/DK]; Danisco Intellectual Capital Langebrogade 1 P.O. Box 17 DK-1001 Copenhagen K, DK (AllExceptUS)
GENENCOR INTERNATIONAL, INC. [US/US]; 925 Page Mill Road Palo Alto, CA 94303, US (AllExceptUS)
BERG, Casper, Tune [DK/DK]; DK (UsOnly)
DERKX, Patrick, Maria, Franciscus [NL/DK]; DK (UsOnly)
FIORESI, Carol [US/US]; US (UsOnly)
GERRITSE, Gijsbert [NL/NL]; NL (UsOnly)
KELLET-SMITH, Anja, Hemmingen [DK/DK]; DK (UsOnly)
KRAGH, Karsten, Matthias [DK/DK]; DK (UsOnly)
LIU, Wei [CN/US]; US (UsOnly)
SHAW, Andrew [GB/US]; US (UsOnly)
SØRENSEN, Bo, Spange [DK/DK]; DK (UsOnly)
THOUDAHL, Charlotte, Refdahl [DK/DK]; DK (UsOnly)
Inventors:
BERG, Casper, Tune; DK
DERKX, Patrick, Maria, Franciscus; DK
FIORESI, Carol; US
GERRITSE, Gijsbert; NL
KELLET-SMITH, Anja, Hemmingen; DK
KRAGH, Karsten, Matthias; DK
LIU, Wei; US
SHAW, Andrew; US
SØRENSEN, Bo, Spange; DK
THOUDAHL, Charlotte, Refdahl; DK
Agent:
KHOO, Chong-Yee; D Young & Co 120 Holborn London EC1N 2DY, GB
Priority Data:
60/697,30207.07.2005US
Title (EN) MODIFIED AMYLASE FROM PSEUDOMONAS SACCHAROPHILIA
(FR) AMYLASE MODIFIEE DE PSEUDOMONAS SACCHAROPHILIA
Abstract:
(EN) We describe a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
(FR) L'invention concerne un polypeptide variant PS4 dérivable d'un polypeptide parent ayant une activité d'amylase, choisi dans le groupe constitué de : (a) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 et 334; (b) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 et 334; (c) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 et 334; et (d) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 et 334; par rapport à la numérotation de position d'une séquence exoamylase pseudomonias saccharophilia représentée par SEQ ID N°1. Les utilisations dudit polypeptide en tant qu'additif alimentaire ou pour rations animales, et les acides nucléiques les codant sont également décrits.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
NZ564465MXMX/a/2008/000374KR1020080023746BRPI0612288EP1907538JP2008544751
CN101238210CA2614274AU2006268418IN76/DELNP/2008