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1. (WO2007006572) MEANS AND METHODS FOR DISPLAYING POLYPEPTIDES ON CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2007/006572    International Application No.:    PCT/EP2006/006845
Publication Date: 18.01.2007 International Filing Date: 12.07.2006
IPC:
C12N 15/10 (2006.01), C12N 15/62 (2006.01), C12N 1/21 (2006.01), C12N 1/06 (2006.01)
Applicants: NASCACELL TECHNOLOGIES AG. [DE/DE]; 3. OG, Modul D, Max-Lebsche-Platz 31, 81377 Munich (DE) (For All Designated States Except US).
ADAMS, Thorsten [DE/DE]; (DE) (For US Only).
KOLMAR, Harald [DE/DE]; (DE) (For US Only)
Inventors: ADAMS, Thorsten; (DE).
KOLMAR, Harald; (DE)
Agent: BOHMANN, Armin, K.; Bohmann & Loosen, Nymphenburger Str. 1, 80335 München (DE)
Priority Data:
05015135.6 12.07.2005 EP
Title (EN) MEANS AND METHODS FOR DISPLAYING POLYPEPTIDES ON CELLS
(FR) MOYENS ET PROCEDES PERMETTANT D'AFFICHER DES POLYPEPTIDES SUR DES CELLULES
Abstract: front page image
(EN)Described is a method for presenting a polypeptide of interest on the surface of cells comprising growing and partially lysing cells which express said polypeptide of interest. Also described are methods for identifying cell clones expressing a polypeptide with a desired property and methods for identifying the nucleotide sequences encoding such a polypeptide. Furthermore, gram-negative bacterial cells are described which contain genetic information for expressing the outer membrane protein and intimin and optionally for expressing a protein of interest.
(FR)L'invention concerne un procédé permettant de présenter un polypeptide étudié sur la surface de cellules et consistant à développer et à lyser au moins partiellement des cellules exprimant le polypeptide étudié. L'invention concerne également des procédés d'identification de clones de cellules exprimant un polypeptide doté d'une propriété recherchée et des procédés permettant d'identifier les séquences nucléotidiques codant un tel polypeptide. De plus, l'invention concerne des cellules bactérienne gram négatif renfermant des informations génétiques permettant d'exprimant la protéine de la membrane externe et l'intimine et éventuellement d'exprimer une protéine étudiée.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)