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1. (WO2007005882) METHODS AND COMPOSITIONS FOR EXPRESSING PROTEINS IN PLANTS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/005882 International Application No.: PCT/US2006/026061
Publication Date: 11.01.2007 International Filing Date: 03.07.2006
IPC:
C12N 15/82 (2006.01) ,A01H 5/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
Applicants:
NORTH CAROLINA STATE UNIVERSITY [US/US]; 2401 Research Drive Ncsu Raleigh, North Carolina 27695, US (AllExceptUS)
WEISSINGER, Arthur [US/US]; US (UsOnly)
AZHAKANANDAM, Kasi [IN/US]; US (UsOnly)
QU, Rongda [US/US]; US (UsOnly)
NICHOLSON LEVIN, Jennifer [US/US]; US (UsOnly)
WEISSINGER, Sandra, M. [US/US]; US (UsOnly)
Inventors:
WEISSINGER, Arthur; US
AZHAKANANDAM, Kasi; US
QU, Rongda; US
NICHOLSON LEVIN, Jennifer; US
WEISSINGER, Sandra, M.; US
Agent:
MERCHANT, Mary, Anthony; Troutman Sanders LLP Bank of America Plaza 600 Peachtree Street, N.E. Suite 5200 Atlanta, GA 30308-2216, US
Priority Data:
60/696,77305.07.2005US
Title (EN) METHODS AND COMPOSITIONS FOR EXPRESSING PROTEINS IN PLANTS
(FR) METHODE ET COMPOSITIONS D'EXPRESSION DE PROTEINES DANS DES PLANTES
Abstract:
(EN) Methods and compositions comprising an expression construct and a suppressor of posttranscriptional gene silencing construct are described. The expression construct and suppressor construct may comprise a viral amplicon. The expression construct may comprise fusing the target gene to the 3’ and/or 5’ end of a gene encoding a transit peptide sequence or a signaling peptide sequences. The transit or signal peptide sequence directs the target gene product to a subcellular location. Methods comprise the production of several heterologous proteins in a single plant. The invention comprises methods for plant production and protein harvest that will yield useful amounts of the desired protein(s) in as little as one to two weeks after the initiation of the production cycle. Methods for the inoculation of recipient plants by spraying with recombinant Agrobacterium suspensions containing the constructs of interest are taught.
(FR) L'invention concerne des méthodes et des compositions contenant un produit de synthèse d'expression et un suppresseur de produit de synthèse de blocage de gène post-transcriptionnel. Le produit de synthèse d'expression et le produit de synthèse suppresseur peuvent comprendre un amplicon viral. Le produit de synthèse d'expression peut comprendre la fusion du gène cible à l'extrémité 3' et/ou 5' d'un gène codant pour une séquence peptidique de transit ou une séquence peptidique de signalisation. La séquence peptidique de transit ou de signalisation dirige le produit génique cible vers un emplacement sous-cellulaire. Les méthodes comprennent la production de plusieurs protéines hétérologues dans une seule plante. L'invention concerne également des méthodes de production de plantes et de récolte de protéines permettant de produire des quantités utiles de la ou des protéines désirées en une à deux semaines après le début du cycle de production. L'invention concerne en outre des méthodes d'inoculation dans des plantes réceptrices par pulvérisation de suspensions d'Agrobacterium de recombinaison contenant lesdits poroduits de synthèse.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
US20110138495