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1. (WO2007005649) PROXIMITY PROBING OF TARGET PROTEINS COMPRISING RESTRICTION AND/OR EXTENSION FIELD
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/005649 International Application No.: PCT/US2006/025660
Publication Date: 11.01.2007 International Filing Date: 30.06.2006
IPC:
C12P 19/34 (2006.01) ,C07H 21/04 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
H
SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
04
with deoxyribosyl as saccharide radical
Applicants:
APPLERA CORPORATION [US/US]; 850 Lincoln Centre Drive Foster City, California 94404, US (AllExceptUS)
ANDERSON, Mark, R. [US/US]; US (UsOnly)
SCHROEDER, Benjamin, G. [US/US]; US (UsOnly)
LAO, Kai, Qin [US/US]; US (UsOnly)
Inventors:
ANDERSON, Mark, R.; US
SCHROEDER, Benjamin, G.; US
LAO, Kai, Qin; US
Agent:
FINN, Andrew, K. ; 850 Lincoln Centre Drive Foster City, California 94404, US
Priority Data:
60/696,10830.06.2005US
Title (EN) PROXIMITY PROBING OF TARGET PROTEINS COMPRISING RESTRICTION AND/OR EXTENSION FIELD
(FR) SONDAGE DE PROXIMITE DE PROTEINES CIBLES COMPRENANT UN CHAMP DE RESTRICTION ET/OU D'EXTENSION
Abstract:
(EN) The present teachings provide methods, compositions, and kits for detecting target analytes, including proteins. In some embodiments, cleavage reactions are performed in the context of proximity probe reactions that query target proteins, wherein the presence and/or quantity of cleavage products is indicative of the presence and/or quantity of a target protein. In some embodiments, the cleavage fragments are quantitated using a real time PCR assay comprising a stem-loop primer, wherein the stem-loop primer comprises a self-complementary hairpin structure and a free 3'end end complementary to the cleavage product. In some embodiments, polymerase extension approaches are employed in the context of proximity probe reactions.
(FR) Des procédés, des compositions et des kits de détection de substances cibles à analyser, y compris les protéines, impliquent des réactions de scission dans le contexte de réactions de sonde à proximité nécessitant des protéines cibles. La présence et/ou la quantité de produits de scission indique la présence et/ou la quantité d'une protéine cible. Selon certaines variantes, les fragments de clivage sont quantifiés au moyen d'un essai par amplification en chaîne par polymérase en temps réel comprenant une amorce tige/boucle comprenant une structure boucle en épingle à cheveux autocomplémentaire et une terminaison 3' libre complémentaire au produit de scission. Selon certaines variantes, les techniques d'extension de polymérase sont utilisées dans le contexte de réaction de sonde à proximité.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)