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1. (WO2007004325) PROTEIN PREPARATION CONTAINING S1-5
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2007/004325 International Application No.: PCT/JP2005/024137
Publication Date: 11.01.2007 International Filing Date: 28.12.2005
IPC:
C12N 15/09 (2006.01) ,A01K 67/027 (2006.01) ,A61K 38/00 (2006.01) ,A61P 19/10 (2006.01) ,A61P 43/00 (2006.01) ,C07K 14/435 (2006.01) ,C07K 16/18 (2006.01) ,C12P 21/02 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K
ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67
Rearing or breeding animals, not otherwise provided for; New breeds of animals
027
New breeds of vertebrates
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38
Medicinal preparations containing peptides
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
19
Drugs for skeletal disorders
08
for bone diseases, e.g. rachitism, Paget's disease
10
for osteoporosis
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
P
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
43
Drugs for specific purposes, not provided for in groups A61P1/-A61P41/108
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
Applicants:
株式会社ロコモジェン LOCOMOGENE, INC. [JP/JP]; 〒1050001 東京都港区虎ノ門4丁目1番地1号 虎ノ門パストラル本館7階 Tokyo #400, Leading Venture Plaza-2 75-1 Ono-cho, Tsurumi-ku Yokohama-shi, Kanagawa 230-0046, JP (AllExceptUS)
中島 利博 NAKAJIMA, Toshihiro [JP/JP]; JP (UsOnly)
八木下 尚子 YAGISHITA, Naoko [JP/JP]; JP (UsOnly)
天野 徹也 AMANO, Tetsuya [JP/JP]; JP (UsOnly)
Inventors:
中島 利博 NAKAJIMA, Toshihiro; JP
八木下 尚子 YAGISHITA, Naoko; JP
天野 徹也 AMANO, Tetsuya; JP
Agent:
清水 初志 SHIMIZU, Hatsushi; 〒3000847 茨城県土浦市卸町1-1-1 関鉄つくばビル6階 Ibaraki Kantetsu Tsukuba Bldg. 6F 1-1-1, Oroshi-machi Tsuchiura-shi Ibaraki3000847, JP
Priority Data:
PCT/JP2005/01225101.07.2005JP
Title (EN) PROTEIN PREPARATION CONTAINING S1-5
(FR) PREPARATION PROTEINIQUE CONTENANT S1-5
(JA) S1-5を含有するタンパク質製剤
Abstract:
(EN) It is found that a knockout mouse having a lost S1-5 gene function can induce an aging-related disease or condition. In the knockout mouse, it is found that the bone mineral content, the bone density and the bone strength are reduced and that the number of osteoclasts in the bone tissue is increased. As a result of the analysis on the ability to form osteoclasts using bone marrow cells derived from the knockout mouse, it is found that the knockout mouse is increased in the ability to form osteoclasts and also is increased in size of osteoclasts compared to a wild-type mouse. When purified S1-5 protein is added to the in vitro system, it is found that the ability to form osteoclasts is reduced. Further, when purified S1-5 protein is administered to an osteoporosis model mouse, it is found that the S1-5 protein can ameliorate osteoporosis. These findings indicate that S1-5 protein is useful for the treatment or prevention of an aging-related disease such as osteoporosis.
(FR) L’invention concerne la découverte selon laquelle une souris knock-out présentant une perte de la fonction du gène S1-5 peut induire une maladie ou un état lié au vieillissement. Dans la souris knock-out, on a découvert que la teneur minérale osseuse, la densité osseuse et la résistance osseuse étaient réduites et que le nombre d’ostéoclastes dans le tissu osseux était plus élevé. De l’analyse de la capacité à former des ostéoclastes en utilisant des cellules de moelle osseuse dérivées de la souris knock-out, il ressort que la souris knock-out présente une capacité plus importante à former des ostéoclastes, ainsi qu’une taille accrue d’ostéoclastes par rapport à une souris de type sauvage. Lorsqu’une protéine S1-5 purifiée est ajoutée dans le système in vitro, on a découvert que la capacité à former des ostéoclastes était réduite. En outre, lorsqu’une protéine S1-5 purifiée est administrée à une souris modèle de l’ostéoporose, on a découvert que la protéine S1-5 pouvait améliorer l’ostéoporose. Ces résultats indiquent que la protéine S1-5 est utile pour le traitement ou la prévention d’une maladie liée au vieillissement, telle que l’ostéoporose.
(JA)  S1-5遺伝子の機能を失わせたノックアウトマウスが、加齢性の疾患又は症状を引き起こすことを見出した。また、該ノックアウトマウスの骨塩量、骨密度、骨強度が低下していること、並びに骨組織において破骨細胞数が増加していることが判明した。また、該ノックアウトマウス由来骨髄細胞を用いた破骨細胞形成能の解析を行った結果、該ノックアウトマウスでは、野生型マウスに比べ、破骨細胞形成能が亢進し、さらに破骨細胞の大きさも大きくなっていることが判明した。また、このin vitroの系に、精製したS1-5タンパク質を添加したところ、破骨細胞形成能が抑制されることが判明した。さらに、骨粗鬆症モデルマウスに、精製したS1-5タンパク質を投与したところ、骨粗鬆症改善効果を有することが判明した。以上の知見は、S1-5タンパク質が、骨粗鬆症などの加齢性の疾患の治療や予防に役立つことを示すものである。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
KR1020080032139EP1764413JPWO2007004325US20100058488CN101258240CA2613813
AU2005333933IN842/DELNP/2008