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1. WO2006125458 - PARALLEL SEQUENCING OF TRANSFORMED NUCLEIC ACIDS IN ENCAPSULATED CELLS

Publication Number WO/2006/125458
Publication Date 30.11.2006
International Application No. PCT/EP2005/005768
International Filing Date 27.05.2005
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12N 11/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
04entrapped within the carrier, e.g. gel or hollow fibres
CPC
C12N 11/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
04entrapped within the carrier, e.g. gel or hollow fibres
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
Applicants
  • ETH ZÜRICH [CH]/[CH] (AllExceptUS)
  • HELD, Martin [DE]/[CH] (UsOnly)
  • LEVIEV, Ilia [RU]/[CH] (UsOnly)
  • MAGYAR, Josef, P. [CH]/[CH] (UsOnly)
  • PANKE, Sven [DE]/[CH] (UsOnly)
  • PELLAUX, Rene [CH]/[CH] (UsOnly)
  • REINOSO-GARCIA, Marta, Maria [ES]/[CH] (UsOnly)
  • SILVERBERG, Eve [GB]/[CH] (UsOnly)
  • WALSER, Marcel [CH]/[CH] (UsOnly)
Inventors
  • HELD, Martin
  • LEVIEV, Ilia
  • MAGYAR, Josef, P.
  • PANKE, Sven
  • PELLAUX, Rene
  • REINOSO-GARCIA, Marta, Maria
  • SILVERBERG, Eve
  • WALSER, Marcel
Agents
  • KASCHE, André
Priority Data
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PARALLEL SEQUENCING OF TRANSFORMED NUCLEIC ACIDS IN ENCAPSULATED CELLS
(FR) SEQUENÇAGE PARALLELE D'ACIDES NUCLEIQUES TRANSFORMEES DANS DES CELLULES ENCAPSULEES
Abstract
(EN)
The present invention relates to a method for sequencing nucleic acids, comprising the provision of one or more capsules, each comprising one or more identical transformed cell(s) with at least one copy of a transformed nucleic acid therein, lysis of the cell(s) and subsequent sequencing of the transformed nucleic acid(s) in the capsule. A further aspect is directed to the use of these methods for sequencing transformed nucleic acids.
(FR)
L'invention concerne un procédé de séquençage d'acides nucléiques. Ce procédé implique: préparation d'une ou de plusieurs capsules comprenant chacune une ou plusieurs cellules identiques transformées ainsi qu'au moins une copie d'un acide nucléique transformé; lyse des cellules et séquençage des acides nucléiques transformés dans la capsule. Un autre aspect concerne l'utilisation de ces procédés dans le séquençage d'acides nucléiques transformés.
Also published as
EP5754496
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