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1. (WO2006122424) PROTEIN DEPLETION USING VOLATILE BINDING BUFFERS

Pub. No.:    WO/2006/122424    International Application No.:    PCT/CA2006/000821
Publication Date: Nov 23, 2006 International Filing Date: May 19, 2006
IPC: C07K 1/22
C07K 1/02
G01N 33/483
G01N 33/50
G01N 33/53
Applicants: CAPRION PHARMACEUTICALS INC.
SITNIKOV, Dmitri
THIBAUDEAU, Eric
KONDEJEWSKI, Leslie, Henry
Inventors: SITNIKOV, Dmitri
THIBAUDEAU, Eric
KONDEJEWSKI, Leslie, Henry
Title: PROTEIN DEPLETION USING VOLATILE BINDING BUFFERS
Abstract:
Methods have been developed for purifying a sample by protein depletion that comprise a step of incorporating a volatile binding buffer into a biological sample prior to protein depletion The depleted sample can then be desalted in situ by vacuum evaporation, thereby increasing sample compatibility with post-depletion processing techniques while maintaining sample integrity, as compared with more labour intensive desalting methods such as dialysis, ultrafiltration, and size-exclusion The methods are useful in applications requiring protein or peptide depletion techniques, such as lmmuno-affimty depletion in sample processing for medical diagnostics, industrial purification, or proteomics