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Pub. No.:    WO/2006/121165    International Application No.:    PCT/JP2006/309598
Publication Date: 16.11.2006 International Filing Date: 12.05.2006
Chapter 2 Demand Filed:    28.11.2006    
C12P 21/02 (2006.01), C12N 9/16 (2006.01), C12N 15/63 (2006.01), C12R 1/01 (2006.01)
Applicants: AJINOMOTO CO., INC. [JP/JP]; 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315 (JP) (For All Designated States Except US).
ITAYA, Hiroshi [JP/JP]; (JP) (For US Only).
KIKUCHI, Yoshimi [JP/JP]; (JP) (For US Only).
DATE, Masayo [JP/JP]; (JP) (For US Only)
Inventors: ITAYA, Hiroshi; (JP).
KIKUCHI, Yoshimi; (JP).
DATE, Masayo; (JP)
Agent: KAWAGUCHI, Yoshiyuki; Acropolis 21 Building 6th floor 4-10, Higashi Nihonbashi 3-chome Chuo-ku, Tokyo 1030004 (JP)
Priority Data:
2005-139798 12.05.2005 JP
(JA) タンパク質の製造法
Abstract: front page image
(EN)By culturing a methanol-assimilating bacterium having a DNA construct containing a promoter sequence which functions in the methanol-assimilating bacterium and a nucleotide sequence encoding a polypeptide containing a signal sequence and an objective protein sequence, which are connected to the promoter sequence such that they can be expressed, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.
(FR)En cultivant une bactérie assimilant le méthanol ayant une construction d’ADN contenant une séquence de promoteur qui fonctionne dans la bactérie assimilant le méthanol et une séquence nucléotidique codant pour un polypeptide contenant une séquence signal et une séquence de protéine object, qui sont reliées à la séquence de promoteur de telle façon qu’elles peuvent être exprimées, dans un milieu liquide contenant du méthanol comme source principale de carbone, on permet à la bactérie de secréter la protéine object, et on récupère la protéine object secrétée.
(JA) メタノール資化性細菌で機能するプロモーター配列、及び該プロモーター配列に発現可能に連結された、シグナル配列および目的タンパク質配列を含むポリペプチドをコードする核酸配列を含むDNA構築物を保持するメタノール資化性細菌を、メタノールを主要炭素源とする液体培地中で培養することにより、該細菌に目的タンパク質を分泌させ、分泌された目的タンパク質を回収する。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)