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1. WO2006119280 - LYOPHILIZED PELLETS

Publication Number WO/2006/119280
Publication Date 09.11.2006
International Application No. PCT/US2006/016814
International Filing Date 03.05.2006
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
F26B 5/06
FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
26DRYING
BDRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
5Drying solid materials or objects by processes not involving the application of heat
04by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
06the process involving freezing
F26B 5/065
FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
26DRYING
BDRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
5Drying solid materials or objects by processes not involving the application of heat
04by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
06the process involving freezing
065the product to be freeze-dried being sprayed, dispersed or pulverised
Applicants
  • HandyLab, Inc. [US]/[US] (AllExceptUS)
  • PHADKE, Nikhil [IN]/[US] (UsOnly)
  • MANENTE, Michelle B. [US]/[US] (UsOnly)
  • HANDIQUE, Kalyan [IN]/[US] (UsOnly)
Inventors
  • PHADKE, Nikhil
  • MANENTE, Michelle B.
  • HANDIQUE, Kalyan
Agents
  • BONE, Richard G. A.
Priority Data
60/737,51916.11.2005US
PCT/US2005/01524503.05.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) LYOPHILIZED PELLETS
(FR) GRANULES LYOPHILISES
Abstract
(EN)
Lyophilized pellets, suitable for use in a microfluidic device, and a method for preparing the same are described. The lyophilized pellets contain various biological reagents, or microparticles, and a cryoprotectant. The lyophilized pellets have a high degree of sphericity and are in the size range 0.5 to 35 µL. The pellets are prepared by dispensing drops of reagent solution on to a cryogenically cooled plate, followed by subjecting to a vacuum.
(FR)
L'invention concerne des granules lyophilisés destinés à être utilisés dans un dispositif microfluidique, ainsi que leur procédé de préparation. Ces granules lyophilisés contiennent divers réactifs biologiques, ou microparticules, ainsi qu'un cryoprotecteur. Ces granules lyophilisés ont un degré de sphéricité élevé et ont un volume compris entre 0,5 et 35 μL. Ces granules sont préparés selon un procédé qui consiste à distribuer des gouttes d'une solution réactive sur une plaque refroidie par voie cryogénique, puis à appliquer le vide.
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