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1. (WO2006119137) METHODS AND COMPOSITIONS FOR REGULATED EXPRESSION OF NUCLEIC ACID AT POST-TRANSCRIPTIONAL LEVEL

Pub. No.:    WO/2006/119137    International Application No.:    PCT/US2006/016514
Publication Date: Nov 9, 2006 International Filing Date: Apr 28, 2006
IPC: C07H 21/02
C07H 21/04
Applicants: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
SAMULSKI, Richard J.
Inventors: SAMULSKI, Richard J.
Title: METHODS AND COMPOSITIONS FOR REGULATED EXPRESSION OF NUCLEIC ACID AT POST-TRANSCRIPTIONAL LEVEL
Abstract:
The present invention provides an isolated nucleic acid comprising: a) at least one first nucleotide sequence encoding a heterologous nucleotide sequence of interest; and b) at least two second heterologous nucleotide sequences, wherein each second heterologous nucleotide sequences comprises: i) a first set of splice elements defining a first intron that is removed by splicing to produce a first RNA molecule that imparts a biological function in the absence of activity at a second set of splice elements; and ii) the second set of splice elements defining one or more introns different from said first intron, wherein said one or more introns different from said firs intron are removed by splicing to produce no RNA molecule and/or a second RNA molecule that does not impart a biological function, when said second set of splice elements is active. Further provided are methods of using the nucleic acid of this invention to regulate transgene expression.