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1. WO2006102491 - ALTERATION OF PLANT EMBRYO/ENDOSPERM SIZE DURING SEED DEVELOPMENT

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CLAIMS
What is claimed is:
1. An isolated polynucleotide comprising:
(a) a nucleic acid sequence encoding a polypeptide involved in altering embryo/endosperm size during seed development, said polypeptide
having at least 80% amino acid sequence identity, based on the Clustal V method of alignment, when compared to an amino acid sequence
selected from the group consisting of SEQ ID NOs:37, 39, 41, 43, 45, 47, 49, 51 , and 53; or
(b) a nucleic acid sequence set forth in SEQ ID NO:25 wherein said
sequence comprises at least one of the following modifications:
(i) nucleotide 271 is a T residue instead of a C;
(ii) nucleotide 110 is a T residue instead of a G; or
(iii) nucleotide 75 is deleted; or
(c) a nucleic acid sequence set forth in SEQ ID NO:34 wherein
(i) nucleotides 4473 through 4829 correspond to a first exon,
and
(ii) nucleotides 5661 through 6110 correspond to a second
exon, and
further wherein the nucleotides of (c) (i) and/or (c)(ii) encode a
polypeptide involved in altering embryo/endosperm size during
seed development; or
(d) a nucleic acid sequence set forth in SEQ ID NO:72; or
(e) a full complement of (a), (b), (c), (d), or SEQ ID NO:34; or
(f) all or part of a non-coding or coding region of the isolated
polynucleotide comprising sequences of (a), (b), (c), (d), (e), or SEQ ID NO:34 for use in co-suppression or antisense suppression of
endogenous nucleic acid sequences encoding polypeptides
involved in altering embryo/endosperm size during seed
development.
2. The isolated polynucleotide of Claim 1 wherein the amino acid sequence identity is at least 85%.

3. The isolated polynucleotide of Claim 1 wherein the amino acid sequence identity is at least 90%.
4. The isolated polynucleotide of Claim 1 wherein the amino acid sequence identity is at least 95%.
5. The isolated polynucleotide of Claim 1 wherein the amino acid sequence identity is 100%.
6. The isolated polynucleotide of Claim 1 wherein the nucleotide sequence corresponds to any of the nucleotide sequences set forth in SEQ ID NOs:34, 36, 38, 40, 42, 44, 46, 48, 50, 52, and 72.
7. A recombinant DNA construct comprising the isolated polynucleotide of any one of Claims 1-6 operably linked to at least one regulatory sequence.
8. A plant comprising in its genome the recombinant DNA construct of Claim 7.
9. Seeds and progeny thereof obtained from the plant of Claim 8.
10. Oil obtained from the seeds of Claim 9.
11. The plant of Claim 8 wherein said plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.
12. Transformed plant tissue or plant cells comprising the recombinant DNA construct of Claim 7.
13. The transformed plant tissue or plant cells of Claim 12 wherein the plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.
14. A method of altering embryo/endosperm size during seed development in a plant comprising:
(a) transforming plant cells or plant tissue with the recombinant DNA
construct of Claim 7;
(b) regenerating transgenic plants from the transformed plant cells or
plant tissue of (a);
(c) obtaining seeds and progeny thereof from the transgenic plants of
(b) having altered embryo/endosperm size based on a comparison of embryo/endosperm size of seeds obtained from non-transformed plants.

15. The method of Claim 14 wherein said plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.
16. A method of mapping genetic variations related to controlling
embryo/endosperm size and/or altering oil phenotype in plants comprising:
(a) crossing two plant varieties; and
(b) evaluating genetic variations with respect to
(i) a nucleic acid sequence selected from the group consisting of
SEQ ID NOs-,25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52
and 72; or
(ii) a nucleic acid sequence encoding a polypeptide selected from
the group consisting of SEQ ID NOs:26, 29, 31 , 33, 37, 39, 41 , 43, 45, 47, 49, 51, and 53;
in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of RFLP analysis, SNP analysis, and PCR-based analysis.
17. The method of Claim 16 wherein the plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.
18. A method of molecular breeding to control embryo/endosperm size
and/or altering oil phenotype in plants comprising:
(a) crossing two plant varieties; and
(b) evaluating genetic variations with respect to
(i) a nucleic acid sequence selected from the group consisting of
SEQ ID NOs:25, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52 and 72; or
(ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs:26, 29, 31 , 33, 30, 32, 34, 36, 38,
40, 42, 44, and 46;
in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of RFLP analysis, SNP analysis, and PCR-based analysis.

19. The plant of Claim 18 wherein the plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.