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1. WO2006102309 - METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF MICRO RNA

Publication Number WO/2006/102309
Publication Date 28.09.2006
International Application No. PCT/US2006/010193
International Filing Date 21.03.2006
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6809
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6809Methods for determination or identification of nucleic acids involving differential detection
C12Q 1/6816
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
Applicants
  • STRATAGENE [US]/[US] (AllExceptUS)
  • SORGE, Joseph, A. [US]/[US] (UsOnly)
  • MULLINAX, Rebecca, L. [US]/[US] (UsOnly)
Inventors
  • SORGE, Joseph, A.
  • MULLINAX, Rebecca, L.
Agents
  • LATIMER, Matthew, T.
Priority Data
11/084,08221.03.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF MICRO RNA
(FR) METHODES, COMPOSITIONS ET TROUSSES DE DETECTION DE MICRO-ARN
Abstract
(EN)
The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.
(FR)
La présente invention concerne des méthodes, des acides nucléiques, des compositions et des trousses permettant de détecter un micro-ARN (miARN) dans des échantillons. Les méthodes consistent à ligaturer ensemble deux oligonucléotides d'une façon induite par un micro-ARN, puis à détecter le produit de la ligature. Les procédés peuvent également consister à amplifier le produit de la ligature, par exemple par une réaction en chaîne de la polymérase (PCR). Les acides nucléiques, les compositions et les trousses comprennent d'ordinaire certains ou l'ensemble des composants nécessaires à la mise en oeuvre de la méthode de cette invention.
Also published as
JP2008503087
Latest bibliographic data on file with the International Bureau