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1. (WO2006102233) METHOD AND APPARATUS FOR IMAGING TARGET COMPONENTS IN A BIOLOGICAL SAMPLE USING PERMANENT MAGNETS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2006/102233 International Application No.: PCT/US2006/010026
Publication Date: 28.09.2006 International Filing Date: 20.03.2006
Chapter 2 Demand Filed: 20.09.2006
IPC:
G01N 33/553 (2006.01) ,G01N 33/53 (2006.01) ,C02F 1/48 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
551
the carrier being inorganic
553
Metal or metal coated
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
02
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
F
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
1
Treatment of water, waste water, or sewage
48
with magnetic or electric fields
Applicants:
IMMUNIVEST CORPORATION [US/US]; 1105 Market Street Suite 1300 P.O. Box 8985 Wilmington, Delaware 19899, US (AllExceptUS)
TIBBE, Arjan [NL/NL]; NL (UsOnly)
TERSTAPPEN, Leon, W., M., M. [NL/US]; US (UsOnly)
Inventors:
TIBBE, Arjan; NL
TERSTAPPEN, Leon, W., M., M.; US
Agent:
ACETO, Joseph; IMMUNICON CORPORATION Suite 100 3401 Masons Mill Road Huntingdon Valley, PA 19006, US
Priority Data:
60/594,19818.03.2005US
Title (EN) METHOD AND APPARATUS FOR IMAGING TARGET COMPONENTS IN A BIOLOGICAL SAMPLE USING PERMANENT MAGNETS
(FR) PROCEDE ET APPAREIL D'IMAGERIE DE COMPOSANTS CIBLES DANS UN ECHANTILLON BIOLOGIQUE AU MOYEN D'AIMANTS PERMANENTS
Abstract:
(EN) A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labelled, but only the target cells are also magnetically labelled. A small, permanent magnet is inserted directly into the chamber containing the labelled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample.
(FR) L'invention concerne un système de numération de cellules dans des fluides, par cytométrie en image, pour évaluer des populations cibles telles que des sous-groupes de leucocytes dans différents liquides organiques, ou une contamination bactérienne dans des échantillons environnementaux, des produits alimentaires, et des liquides organiques. Pour résumer, l'ensemble des cellules d'un échantillon biologique sont marquées par fluorescence, mais uniquement les cellules cibles sont également marquées de manière magnétique. Un aimant permanent de petite dimension est directement inséré dans la chambre contenant l'échantillon marqué. Les aimants sont enrobés d'un caoutchouc de silicone PDMS de manière à présenter une surface lisse et régulière permettant d'effectuer une imagerie sur un plan focal unique. Les cellules sont éclairées, et les images de la lumière fluorescente émise par les cellules cibles sont capturées par une caméra CCD. L'analyse d'image réalisée au moyen d'un nouvel algorithme permet de fournir le nombre des cellules sur la surface qui peuvent être liées à la concentration de cellules cibles de l'échantillon initial.
front page image
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)