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1. WO2006099030 - METHODS OF ISLET ISOLATION AND TRANSPLANTATION

Publication Number WO/2006/099030
Publication Date 21.09.2006
International Application No. PCT/US2006/008390
International Filing Date 09.03.2006
IPC
C12N 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
C12N 5/071 2010.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
C12Q 1/00 2006.01
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
CPC
A61K 35/12
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
C12N 2500/02
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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2500Specific components of cell culture medium
02Atmosphere, e.g. low oxygen conditions
C12N 5/0676
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0676Pancreatic cells
Applicants
  • MEDIATECH, INC. [US]/[US] (AllExceptUS)
  • DEOLDEN, James [US]/[US] (UsOnly)
  • LAKEY, Jonathan [CA]/[CA] (UsOnly)
Inventors
  • DEOLDEN, James
  • LAKEY, Jonathan
Agents
  • SILVERMAN, Arnold, B.
Priority Data
11/080,79714.03.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS OF ISLET ISOLATION AND TRANSPLANTATION
(FR) METHODES D'ISOLATION ET DE TRANSPLANTATION D'ILOTS
Abstract
(EN)
The present invention relates to methods for improving the viability and recovery of islets that are separated from a donor organ for subsequent transplantation. In a preferred embodiment, the islets are separated from a donor pancreas and transplanted into the liver of a diabetic patient. One or more emulsified perfluorocarbons (ePFCs) may be infused into the donor pancreas prior to islet isolation and transplantation. The ePFCs may enhance the oxygenation of islets, thereby enhancing their viability and health so they may withstand a vigorous isolation procedure such as the Edmonton Protocol. The present invention not only preserves the donor organ using ePFC, but also rescues islets and donor organs that would otherwise be damaged or destroyed during isolation and transplantation procedures. The separated islets may be injected into the portal vein of a liver where they will assist in producing insulin and regulating blood glucose levels.
(FR)
L'invention concerne des méthodes permettant d'améliorer la viabilité et la récupération d'îlots qui sont séparés d'un organe donneur pour une transplantation ultérieure. Dans un mode de réalisation préféré, les îlots sont séparés du pancréas d'un donneur et transplantés dans le foie d'un patient diabétique. On peut perfuser un ou plusieurs perfluorocarbone(s) émulsifié(s) (ePFC) dans le pancréas du donneur avant d'isoler et de transplanter un îlot. Les ePFC permettent d'améliorer l'oxygénation des îlots, ce qui permet d'améliorer leur viabilité et leur santé de sorte qu'ils peuvent supporter une procédure d'isolation vigoureuse, telle que le protocole d'Edmonton. Non seulement l'invention permet de préserver l'organe du donneur à l'aide d'un ePFC, mais elle permet également de sauver des îlots et des organes de donneur qui autrement seraient endommagés ou détruits pendant les procédures d'isolation et de transplantation Les îlots séparés peuvent être injectés dans la veine porte d'un foie dans lequel ils facilitent la production d'insuline et la régulation des taux de glucose sanguin.
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