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1. WO2006099019 - METHODS AND COMPOSITION RELATED TO IN VIVO IMAGING OF GENE EXPRESSION

Publication Number WO/2006/099019
Publication Date 21.09.2006
International Application No. PCT/US2006/008374
International Filing Date 09.03.2006
Chapter 2 Demand Filed 07.03.2007
IPC
C12N 15/85 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C07K 14/705 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
A61K 49/00 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
49Preparations for testing in vivo
CPC
A61K 49/0002
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
49Preparations for testing in vivo
0002General or multifunctional contrast agents, e.g. chelated agents
C12N 15/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 2830/008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
008cell type or tissue specific enhancer/promoter combination
C12N 2830/15
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
15chimeric enhancer/promoter combination
C12N 2830/60
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
60from viruses
C12N 2830/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
80from vertebrates
85mammalian
Applicants
  • BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM [US]/[US] (AllExceptUS)
  • KUNDRA, Vikas [US]/[US] (UsOnly)
  • FANG, Bingliang [CN]/[US] (UsOnly)
  • JI, Lin, X. [US]/[US] (UsOnly)
  • YANG, Dan [CN]/[US] (UsOnly)
Inventors
  • KUNDRA, Vikas
  • FANG, Bingliang
  • JI, Lin, X.
  • YANG, Dan
Agents
  • DE LA PAZ, Monica, A.
Priority Data
60/659,84409.03.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHODS AND COMPOSITION RELATED TO IN VIVO IMAGING OF GENE EXPRESSION
(FR) METHODES ET COMPOSITION RELATIVES A L'IMAGERIE IN VIVO D'UNE EXPRESSION GENIQUE
Abstract
(EN)
Disclosed are nucleic acid sequences encoding a recombinant seven transmembrane G-protein associated receptor (GPCR) amino acid sequence operatively coupled to a tissue-selective promoter sequence. Also disclosed are methods of imaging or treating cells in a subject that involve introducing a nucleic acid encoding a reporter detectable in vivo using non-invasive methods operatively coupled to a tissue-selective promoter or amplified tissue specific promoter to the subject, and subjecting the subject to a non-invasive imaging technique or a therapeutic that selectively interacts with the reporter or gene of interest. For example, the subject may be a subject with cancer, and the therapeutic may be an anti-cancer agent. In particular, the hTERT promoter or amplified hTERT promoter drives expression of a reporter such hSSTR2 and/or hSSTR2 derivative) and these may be linked to a gene of interest. Imaging may be performed by a variety of methods including nuclear medicine.
(FR)
L'invention concerne des séquences d'acides nucléiques codant pour une séquence d'acide aminé du récepteur de recombinaison à sept transmembranes couplé à une protéine G (GPCR), fonctionnellement couplée à une séquence de promoteur sélectif d'un tissu. L'invention concerne également des méthodes d'imagerie ou de traitement de cellules chez un sujet impliquant l'introduction d'un acide nucléique codant pour un reporter détectable in vivo à l'aide de méthodes non invasives couplé à un promoteur sélectif d'un tissu ou à un promoteur sélectif d'un tissu amplifié du sujet, et la soumission dudit sujet à une technique d'imagerie non invasive ou à une thérapeutique qui interagit sélectivement avec le reporter ou le gène d'intérêt. Le sujet peut, par exemple, souffrir d'un cancer, et la thérapeutique peut être un agent anticancéreux. Le promoteur hTERT ou le promoteur hTERT amplifié peut entraîner l'expression d'un reporter, tel que hSSTR2 et/ou un dérivé de hSSTR2, ceux-ci pouvant être liés à un gène d'intérêt. L'imagerie peut être effectuée au moyen d'une variété de méthodes, notamment, la médecine nucléaire.
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