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1. WO2006098428 - METHOD OF LESSENING NONSPECIFIC AMPLIFICATION FROM PRIMER DIMER

Publication Number WO/2006/098428
Publication Date 21.09.2006
International Application No. PCT/JP2006/305372
International Filing Date 17.03.2006
IPC
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
CPC
C12Q 1/6848
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6848characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Applicants
  • 株式会社ダナフォーム KABUSHIKI KAISHA DNAFORM [JP]/[JP] (AllExceptUS)
  • 林崎 良英 HAYASHIZAKI, Yoshihide [JP]/[JP] (UsOnly)
  • 三谷 康正 MITANI, Yasumasa [JP]/[JP] (UsOnly)
  • 加藤 千晶 KATO, Chiaki [JP]/[JP] (UsOnly)
Inventors
  • 林崎 良英 HAYASHIZAKI, Yoshihide
  • 三谷 康正 MITANI, Yasumasa
  • 加藤 千晶 KATO, Chiaki
Agents
  • 奥山尚一 OKUYAMA, Shoichi
Priority Data
2005-07652917.03.2005JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) METHOD OF LESSENING NONSPECIFIC AMPLIFICATION FROM PRIMER DIMER
(FR) METHODE POUR DIMINUER L’AMPLIFICATION NON-SPECIFIQUE D’UNE AMORCE DE DIMERE
(JA) プライマーダイマーからの非特異的増幅を減少させる方法
Abstract
(EN)
It is intended to provide a method of suppressing a nonspecific amplification reaction accompanying primer dimer formation in a nucleic acid amplification reaction characterized by comprising adding a substance capable of binding to the primer dimer to a sample. As the substance capable of binding to the primer dimer, use can be made of a substance capable of recognizing mismatch such as a mismatch-binding protein or a substance having a DNA recombination ability such as a DNA recombinase.
(FR)
L’invention concerne une méthode de suppression de la réaction d’amplification non-spécifique accompagnant la formation d’une amorce de dimère au cours d’une réaction d’amplification d'acide nucléique qui se caractérise par l’ajout à un échantillon d'une substance capable de se lier à l'amorce de dimère. En tant que substance capable de se lier à l’amorce de dimère, l'utilisation d'une substance capable de reconnaître un mésappariement tel qu'une protéine d’association des appariements ou l’utilisation d’une substance pouvant se recombiner génétiquement comme une recombinaison d’ADN est possible.
(JA)
 核酸増幅反応において、プライマーダイマーに結合する物質をサンプルに添加することを特徴とする、プライマーダイマー形成に伴う非特異的な増幅反応を抑制する方法を提供する。プライマーダイマーに結合する物質としては、ミスマッチ結合タンパク質のようなミスマッチ認識能を有する物質や、DNA組換え酵素のようなDNA組換え能を有する物質を用いることができる。
Also published as
EP6729362
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