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1. WO2006096126 - A METHOD, DEVICE AND SYSTEM FOR VOLUMETRIC ENUMERATION OF WHITE BLOOD CELLS.

Publication Number WO/2006/096126
Publication Date 14.09.2006
International Application No. PCT/SE2006/000311
International Filing Date 10.03.2006
Chapter 2 Demand Filed 11.10.2006
IPC
G01N 33/50 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
G01N 15/14 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
G01N 33/49 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
49blood
G06K 9/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G06T 7/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
7Image analysis
CPC
A61B 5/150022
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150015Source of blood
150022for capillary blood or interstitial fluid
A61B 5/15003
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150015Source of blood
15003for venous or arterial blood
A61B 5/150099
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150053for enhanced collection of blood or interstitial fluid at the sample site, e.g. by applying compression, heat, vibration, ultrasound, suction or vacuum to tissue; for reduction of pain or discomfort; Skin piercing elements, e.g. blades, needles, lancets or canulas, with adjustable piercing speed
150061Means for enhancing collection
150099by negative pressure, other than vacuum extraction into a syringe by pulling on the piston rod or into pre-evacuated tubes
A61B 5/150229
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150206Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
150229Pumps for assisting the blood sampling
A61B 5/150343
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150343Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes
A61B 5/150755
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
BDIAGNOSIS; SURGERY; IDENTIFICATION
5Detecting, measuring or recording for diagnostic purposes
15Devices for taking samples of blood
150007Details
150755Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
Applicants
  • HEMOCUE AB [SE]/[SE]
  • LINDBERG, Stellan [SE]/[SE] (UsOnly)
  • SVENSSON, Johnny [SE]/[SE] (UsOnly)
Inventors
  • LINDBERG, Stellan
  • SVENSSON, Johnny
Agents
  • AWAPATENT AB
Priority Data
0500549-111.03.2005SE
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A METHOD, DEVICE AND SYSTEM FOR VOLUMETRIC ENUMERATION OF WHITE BLOOD CELLS.
(FR) PROCEDE, DISPOSITIF ET SYSTEME DE NUMERATION VOLUMETRIQUE DE GLOBULES BLANCS
Abstract
(EN)
A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a meas- urement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus . The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.
(FR)
L'invention concerne un dispositif de prélèvement d'échantillon pour la numération volumétrique de globules blancs dans un échantillon de sang comprenant une cavité de mesure destinée à recevoir un échantillon de sang. La cavité de mesure présente une épaisseur fixe prédéterminée. Le dispositif de prélèvement d'échantillon contient également un réactif lequel est placé, sous une forme séchée, sur une surface définissant la cavité de mesure. Le réactif contient un agent d'hémolyse destiné à lyser des globules blancs dans l'échantillon de sang ainsi qu'un agent colorant destiné à colorer sélectivement les globules blancs dans l'échantillon de sang. Un système comprend le dispositif de prélèvement d'échantillon ainsi qu'un appareil de mesure. L'appareil de mesure comprend un support du dispositif de prélèvement d'échantillon, une source de lumière ainsi qu'un système d'imagerie destiné à acquérir une image numérique d'un grossissement de l'échantillon. L'appareil de mesure comprend également un analyseur d'images ménagé pour analyser l'image numérique acquise afin de déterminer le nombre de globules blancs dans l'échantillon de sang.
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