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1. WO2006095480 - CELL CULTURE CHAMBER

Publication Number WO/2006/095480
Publication Date 14.09.2006
International Application No. PCT/JP2005/022524
International Filing Date 08.12.2005
IPC
C12M 3/06 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
CPC
C12M 23/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
23Constructional details, e.g. recesses, hinges
34Internal compartments or partitions
C12M 29/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
29Means for introduction, extraction or recirculation of materials, e.g. pumps
10Perfusion
C12M 35/08
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
35Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
08Chemical, biochemical or biological means, e.g. plasma jet, co-culture
Applicants
  • 財団法人生産技術研究奨励会 The Foundation for The Promotion of Industrial Science [JP]/[JP] (AllExceptUS)
  • 医療法人慈敬会 JIKEIKAI MEDICAL CORPORATION [JP]/[JP] (AllExceptUS)
  • 酒井 康行 SAKAI, Yasuyuki [JP]/[JP] (UsOnly)
  • 藤井 輝夫 FUJII, Teruo [JP]/[JP] (UsOnly)
  • オストロヴィドフ セルジュ OSTROVIDOV, Serge [FR]/[JP] (UsOnly)
  • 乾 裕昭 INUI, Hiroaki [JP]/[JP] (UsOnly)
  • 水野 仁二 MIZUNO, Jinji [JP]/[JP] (UsOnly)
Inventors
  • 酒井 康行 SAKAI, Yasuyuki
  • 藤井 輝夫 FUJII, Teruo
  • オストロヴィドフ セルジュ OSTROVIDOV, Serge
  • 乾 裕昭 INUI, Hiroaki
  • 水野 仁二 MIZUNO, Jinji
Agents
  • 志賀 正武 SHIGA, Masatake
Priority Data
2005-06407408.03.2005JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) CELL CULTURE CHAMBER
(FR) CHAMBRE DE CULTURE CELLULAIRE
(JA) 細胞培養チャンバー
Abstract
(EN)
A cell culture chamber (1) comprising a support (2), a semipermeable membrane (3), an upper compartment (4) and a lower compartment (5) both provided in the inside of the support (2) and partitioned from each other by the semipermeable membrane (3), a culture medium feed flow path (6) for feeding a culture medium into the lower compartment (5), a culture medium discharge flow path (7) for discharging a culture medium in the lower compartment (5), and a perfusion flow path (8) for perfusing a fluid through the upper compartment (4). In the inside of the upper compartment (4), a cell culture section is formed by the sieve structure (9) made of narrow spaces (9a) and walls (9b) through which a fluid can pass but cultured cells cannot. A guide (11) for introducing/collecting cultured cells is connected to the cell culture section.
(FR)
L’invention concerne une chambre de culture cellulaire (1) comprenant un support (2), une membrane semi-perméable (3), un compartiment supérieur (4) et un compartiment inférieur (5) se trouvant tous les deux à l’intérieur du support (2) et séparés par la membrane semi-perméable (3), une voie d’écoulement du milieu de culture de type alimentation (6) qui permet d’alimenter le compartiment inférieur (5) avec un milieu de culture, une voie d’écoulement du milieu de culture de type déchargement (7) qui permet de décharger dans le compartiment inférieur (5) un milieu de culture, et une voie d’écoulement de type perfusion (8) qui permet de perfuser un fluide à travers le compartiment supérieur (4). À l’intérieur du compartiment supérieur (4), la structure en tamis (9), qui forme une section de cellules culture, se compose d’espaces étroits (9a) et de parois (9b) au travers desquels un fluide peut passer mais pas les cellules cultivées. Un guide (11) destiné à introduire/récolter les cellules cultivées est relié à la section des cellules en culture.
(JA)
 細胞培養チャンバー(1)は、支持体(2)の内部に、半透膜(3)によって区画される上部コンパートメント(4)および下部コンパートメント(5)と、下部コンパートメント(5)内に培養液を供給する培養液供給用流路(6)と、下部コンパートメント(5)内の培養液を排出する培養液排出用流路(7)と、上部コンパートメント(4)内に流体を灌流させるための灌流用流路(8)とを有し、上部コンパートメント(4)内には、流体は通過するが培養細胞は通過しない間隙部(9a)と壁部(9b)とから構成されるふるい構造(9)によって、細胞培養部が形成されている。細胞培養部には、培養細胞の導入・回収用のガイド(11)が接続されている。
Also published as
EP5814149
GB0717796.7
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