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1. WO2006095156 - VECTORS COMPRISING NOVEL REGULATORY ELEMENTS

Publication Number WO/2006/095156
Publication Date 14.09.2006
International Application No. PCT/GB2006/000802
International Filing Date 03.03.2006
IPC
C12N 15/85 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
CPC
A61P 37/02
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
37Drugs for immunological or allergic disorders
02Immunomodulators
A61P 5/10
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
5Drugs for disorders of the endocrine system
10of the posterior pituitary hormones, e.g. oxytocin, ADH
A61P 7/04
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
7Drugs for disorders of the blood or the extracellular fluid
04Antihaemorrhagics; Procoagulants; Haemostatatic agents; Antifibrinolytic agents
C12N 15/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
C12N 2830/46
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
46elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
C12N 2840/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2840Vectors comprising a special translation-regulating system
20translation of more than one cistron
Applicants
  • MILLIPORE CORPORATION [US]/[US] (AllExceptUS)
  • WILLIAMS, Steven, Geraint [GB]/[GB] (UsOnly)
  • GAWN, Jonathan [GB]/[GB] (UsOnly)
  • IRVINE, Alistair, Simpson (UsOnly)
Inventors
  • WILLIAMS, Steven, Geraint
  • GAWN, Jonathan
  • IRVINE, Alistair, Simpson
Agents
  • AJELLO, Mike
Priority Data
0504587.705.03.2005GB
60/661,55314.03.2005US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) VECTORS COMPRISING NOVEL REGULATORY ELEMENTS
(FR) VECTEURS COMPRENANT DES NOUVEAUX ELEMENTS REGULATEURS
Abstract
(EN)
The invention relates to the field of recombinant DNA technology and, in particular, the development of vectors for the expression of recombinant proteins. Expression of heterologous genes in eukaryotic cells requires transcription by RNA polymerase II, which is driven by c/s-acting genetic elements known as promoters and enhancers. The invention provides promoters derived from the guinea pig cytomegalovirus early- immediate promoter / enhancer, useful for obtaining high levels of expression of recombinant proteins. Also disclosed are eukaryotic expression vectors comprising such promoters, which are capable of providing increased levels of expression over that obtainable from human or murine cytomegalovirus enhancer /promoter elements in many cell types.
(FR)
L'invention concerne le domaine de la technologie d'ADN recombinant et, plus précisément, le développement de vecteurs destinés à l'expression de protéines recombinantes. L'expression de gènes hétérologues dans des cellules eucaryotes nécessite une transcription par une polymérase II d'ARN, entraînée par des éléments génétiques à action c/s connus comme promoteurs et amplificateurs. L'invention concerne également des promoteurs dérivés des promoteur/amplificateur précoce-immédiat du cytomégalovirus de cobayes, utiles dans l'obtention de niveaux élevés d'expression de protéines recombinantes. L'invention concerne enfin des vecteurs d'expression eucaryotes comprenant de tels promoteurs capables de fournir des niveaux améliorés d'expression par rapport à ceux pouvant être obtenus à partir d'éléments d'amplificateur/promoteur du cytomégavirus humain ou murin dans plusieurs types de cellules.
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