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1. WO2006093330 - METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID

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[ EN ]

CLAIMS
1. A method for separating and purifying nucleic acid, the method comprising:
(1) a step of contacting a sample solution
containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase/
(2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and
(3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase,
wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.

2. The method for separating and purifying nucleic acid according to claim 1,
wherein the surfactant is a nonionic surfactant.

3. The method for separating and purifying nucleic acid according to claim 1 or 2,
wherein the surfactant is a polyoxyethylene
surfactant.

4. The method for separating and purifying nucleic acid according to any of claims 1 to 3,
wherein the surfactant is a polyoxyethylene sorbitan surfactant.

5. The method for separating and purifying nucleic acid according to any of claims 1 to 4,
wherein the sample solution is prepared by adding a pre-treating solution containing at least one selected from a chaotropic salt, a defoaming agent, a nucleic acid stabilizer, a buffer, an acid, an alkali agent and an enzyme to a sample containing nucleic acid.

6. The method for separating and purifying nucleic acid according to any of claims 1 to 5,
wherein the solid phase is a membrane-shaped solid phase .

7. The method for separating and purifying nucleic acid according to any of claims 1 to 6,
wherein the water-soluble organic solvent contains at least one selected from methanol, ethanol, propaήol and its isomer and butanol and its isomer.

8. The method for separating and purifying nucleic acid according to any of claims 1 to 7,
wherein the solid phase contains silica or its derivative, diatomaceous earth or alumina.

9. The method for separating and purifying nucleic acid according to any of claims 1 to 8,
wherein the solid phase contains an organic polymer.

10. The method for separating and purifying nucleic acid according to claim 9,
wherein the solid phase contains at least one selected from Teflon (registered trademark) , a polyester, a polyether sulfone, a polycarbonate, a polyacrylate copolymer, a polyurethane, a polybenzimidazole, a
polyolefin, a polyvinyl chloride and a polyvinylidene fluoride.

11. The method for separating and purifying nucleic acid according to claim 9,
wherein the solid phase contains nylon having positive or negative charges.

12. The method for separating and purifying nucleic acid according to claim 9, wherein the organic polymer has a polysaccharide structure.

13. The method for separating and purifying nucleic acid according to claim 9,
wherein the organic polymer contains at least one selected from cellulose, cellulose mixed ester, cellulose nitrate, cellulose acetate and nitrocellulose.

14. An apparatus for automatically conducting the steps in a method for separating and purifying nucleic acid according to any of claims 1 to 13.

15. A kit for conducting a method for separating and purifying nucleic acid according to any of claims 1 to 13, the kit comprising:
(i) a cartridge for separation and purification of nucleic acid;
(ii) a surfactant;
(iii) a pre-treating solution containing at least one selected from a chaotropic salt, a defoaming agent, a nucleic acid stabilizer, a buffer, an acid, an alkali agent and an enzyme;
(iv) a washing solution; and
(v) a reagent of a recovering solution.