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1. WO2006093325 - METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID

Publication Number WO/2006/093325
Publication Date 08.09.2006
International Application No. PCT/JP2006/304515
International Filing Date 02.03.2006
IPC
C12N 15/09 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12M 1/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
CPC
C12N 15/1006
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
1006by means of a solid support carrier, e.g. particles, polymers
Applicants
  • FUJIFILM Corporation [JP]/[JP] (AllExceptUS)
  • TAKESHITA, Yumiko (UsOnly)
  • KOMAZAWA, Hiroyuki (UsOnly)
  • WATANABE, Shin-ichi (UsOnly)
Inventors
  • TAKESHITA, Yumiko
  • KOMAZAWA, Hiroyuki
  • WATANABE, Shin-ichi
Agents
  • OGURI, Shohei
Priority Data
2005-06063304.03.2005JP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR SEPARATING AND PURIFYING NUCLEIC ACID
(FR) MÉTHODE DE SÉPARATION ET DE PURIFICATION D'UN ACIDE NUCLÉIQUE
Abstract
(EN)
A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of adding at least two enzymes.
(FR)
La présente invention a pour objet une méthode de séparation et de purification d'un acide nucléique, ladite méthode comprenant : (1) une étape de mise en contact d'une solution échantillon contenant un acide nucléique avec une phase solide, afin d'adsorber l'acide nucléique sur la phase solide ; (2) une étape de mise en contact d'une solution de rinçage avec la phase solide afin de rincer la phase solide dans un état òu celle-ci absorbe l'acide nucléique; et (3) une étape de mise en contact d'une solution de récupération avec la phase solide afin de désorber l'acide nucléique de la phase solide, la solution échantillon étant préparée en incluant une étape d'addition d'au moins deux enzymes.
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