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1. WO2006092475 - METHOD FOR THE RELATIVE MEASUREMENT OF THE FLUORESCENCE QUANTUM EFFICIENCY OF DYES IN SOLUTION

Publication Number WO/2006/092475
Publication Date 08.09.2006
International Application No. PCT/FR2005/050896
International Filing Date 25.10.2005
Chapter 2 Demand Filed 11.08.2006
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
CPC
G01N 21/643
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
643non-biological material
Applicants
  • COMMISSARIAT A L'ENERGIE ATOMIQUE [FR]/[FR] (AllExceptUS)
  • BLEUSE, Joël [FR]/[FR] (UsOnly)
  • CARAYON, Sophie [FR]/[FR] (UsOnly)
  • DELAMADELEINE, Eric [FR]/[FR] (UsOnly)
Inventors
  • BLEUSE, Joël
  • CARAYON, Sophie
  • DELAMADELEINE, Eric
Agents
  • LEHU, Jean
Priority Data
045244326.10.2004FR
Publication Language French (FR)
Filing Language French (FR)
Designated States
Title
(EN) METHOD FOR THE RELATIVE MEASUREMENT OF THE FLUORESCENCE QUANTUM EFFICIENCY OF DYES IN SOLUTION
(FR) METHODE DE MESURE RELATIVE DU RENDEMENT QUANTIQUE DE FLUORESCENCE DE COLORANTS EN SOLUTION
Abstract
(EN)
The invention relates to a method for the measurement of the relative fluorescence quantum efficiency of a dye, comprising the following steps, namely: a) for at least two different concentrations of the dye in solution in a solvent, the excitation of the dye with electromagnetic radiation (6) and the measurement of the photoluminescence (67) of the dye that has been excited by said radiation and of the signal transmitted (65) through the cell containing the dye; b) a comparison of the data measured with the photoluminescence and transmission data of a reference dye; and c) the calculation of the relative fluorescence quantum efficiency of the dye.
(FR)
L'invention concerne un procédé de mesure du rendement quantique relatif de fluorescence d'un colorant, comportant : a) pour au moins deux concentrations différentes du colorant en solution dans un solvant : - l'excitation du colorant, à l'aide d'un rayonnement électromagnétique (6) , - la mesure de la photoluminescence (67) du colorant excité par ce rayonnement et du signal transmis (65) à travers la cellule contenant ce colorant, b) la comparaison des données mesurées avec des données de photoluminescence et de transmission d'un colorant de référence, c) le calcul du rendement quantique relatif de fluorescence du colorant.
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