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1. (WO2006090987) METHODS FOR PERFORMING DIRECT ENZYMATIC REACTIONS INVOLVING NUCLEIC ACID MOLECULES
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/090987    International Application No.:    PCT/KR2006/000457
Publication Date: 31.08.2006 International Filing Date: 08.02.2006
IPC:
C12Q 1/68 (2006.01)
Applicants: BIOQUEST, INC. [KR/KR]; #403 Trizm Bldg., 46-1, Hawolgok 2-dong, Seongbuk-gu, Seoul 136-132 (KR) (For All Designated States Except US).
YANG, Young Geun [KR/KR]; (KR) (For US Only).
KIM, Jong Yeol [KR/KR]; (KR) (For US Only).
KIM, Suhng Wook [KR/KR]; (KR) (For US Only).
LEE, Sung Gwan [KR/KR]; (KR) (For US Only)
Inventors: YANG, Young Geun; (KR).
KIM, Jong Yeol; (KR).
KIM, Suhng Wook; (KR).
LEE, Sung Gwan; (KR)
Agent: YANG, Boo-hyun; 4th Fl. Oksan Bldg., 1627-10, Bongcheon-7-dong, Gwanak-gu, Seoul 151-818 (KR)
Priority Data:
10-2005-0016481 28.02.2005 KR
Title (EN) METHODS FOR PERFORMING DIRECT ENZYMATIC REACTIONS INVOLVING NUCLEIC ACID MOLECULES
(FR) METHODES D'EXECUTION DE REACTIONS ENZYMATIQUES DIRECTES METTANT EN OEUVRE DES MOLECULES D'ACIDE NUCLEIQUE
Abstract: front page image
(EN)The present invention relates to methods for performing a direct enzymatic reaction involving a nucleic acid molecule, which comprises the steps of: (a) performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, wherein a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction; and (b) obtaining a product from the enzymatic reaction.
(FR)La présente invention concerne des méthodes d'exécution d'une réaction enzymatique directe mettant en oeuvre une molécule d'acide nucléique, laquelle comprend les étapes consistant: (a) à exécuter la réaction enzymatique directement à l'aide d'un échantillon biologique dans un mélange de réaction contenant un tampon zwitterionique et/ou un glucide non réducteur afin d'empêcher que l'échantillon biologique n'inhibe la réaction enzymatique, la molécule d'acide nucléique présente dans l'échantillon biologique n'est pas purifiée avant la réaction enzymatique; et (b) à obtenir un produit de la réaction enzymatique.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)