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1. (WO2006090696) METHOD FOR PREPARING EPITHELIUM-DENUDED AMNION
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/090696    International Application No.:    PCT/JP2006/303050
Publication Date: 31.08.2006 International Filing Date: 21.02.2006
IPC:
A61L 27/00 (2006.01), A61L 15/64 (2006.01)
Applicants: ARBLAST CO., LTD. [JP/JP]; Kobe International Business Center Bldg. 5-5-2, Minatojima-minamimachi Chuo-ku, Kobe-city, Hyogo 6500047 (JP) (For All Designated States Except US).
KURIHARA, Eiji [JP/JP]; (JP) (For US Only).
HAMURO, Junji [JP/JP]; (JP) (For US Only)
Inventors: KURIHARA, Eiji; (JP).
HAMURO, Junji; (JP)
Agent: KONISHI, Tomimasa; 7F, Marunouchi Estate Bldg. 17-12, Marunouchi 2-chome Naka-ku, Nagoya-shi, Aichi 4600002 (JP)
Priority Data:
2005-046296 22.02.2005 JP
Title (EN) METHOD FOR PREPARING EPITHELIUM-DENUDED AMNION
(FR) PROCÉDÉ DE FABRICATION D’UN AMNION DÉNUÉ D’ÉPITHÉLIUM
(JA) 上皮が除去された羊膜を調製する方法
Abstract: front page image
(EN)One of the objects is to realize the complete denudation of amniotic epithelium by a simple operation with as little damage to the compact layer and basal membrane as possible. Another object is to provide a method capable of treating a number of amnions simultaneously. A method for preparing an epithelium-denuded amnion comprising the following steps of (1) preparing an amnion isolated from a living body; (2) subjecting the amnion to a freezing and thawing treatment; (3) treating the amnion subjected to the freezing and thawing treatment with trypsin; and (4) washing the amnion subjected to the trypsin treatment is provided.
(FR)L’un des objets de l’invention est la réalisation de la dénudation complète d’épithélium amniotique par une opération simple causant le minimum de dégâts possible à la couche compact et à la membrane basale. Un autre objet est de proposer un procédé capable de traiter un certain nombre d’amnions simultanément. Le procédé de fabrication d’un amnion dénué d’épithélium comprend les phases suivantes : (1) préparation d’un amnion isolé d’un corps vivant ; (2) soumission de l’amnion à un traitement de congélation et de décongélation ; (3) traitement de l’amnion soumis au traitement de congélation et de décongélation avec de la trypsine ; et (4) lavage de l’amnion soumis au traitement par trypsine.
(JA) 緻密層及び基底膜の損傷をできるだけ抑えつつ羊膜上皮を完全に除去することを簡便な操作で実現することを目的の一つとする。また、同時に多数の羊膜を処理することが可能な方法を提供することを目的の一つとする。以下のステップを含む、上皮が除去された羊膜を調製する方法が提供される:(1)生体より分離された羊膜を用意するステップ;(2)前記羊膜に凍結融解処理を施すステップ;(3)凍結融解処理後の羊膜にトリプシン処理を施すステップ;及び(4)トリプシン処理後の羊膜を洗浄するステップ。
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)