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Pub. No.:    WO/2006/089259    International Application No.:    PCT/US2006/005903
Publication Date: 24.08.2006 International Filing Date: 17.02.2006
C12Q 1/68 (2006.01), C12N 15/10 (2006.01)
Applicants: AMBION, INC. [US/US]; 2130 Woodward St., No. 200, Austin, TX 78744-1832 (US) (For All Designated States Except US).
LATHAM, Gary [US/US]; (US) (For US Only).
PASLOSKE, Brittan, L. [CA/US]; (US) (For US Only).
PELTIER, Heidi [AU/US]; (US) (For US Only)
Inventors: LATHAM, Gary; (US).
PASLOSKE, Brittan, L.; (US).
PELTIER, Heidi; (US)
Agent: WILSON, Mark, B.; FULBRIGHT & JAWORSKI L.L.P., 600 Congress Avenue, Suite 2400, Austin, TX 78701 (US)
Priority Data:
60/654,219 18.02.2005 US
11/076,455 09.03.2005 US
Abstract: front page image
(EN)The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.
(FR)La présente invention concerne une composition et un procédé permettant d'isoler un acide nucléique d'un échantillon contenant des cellules. L'invention concerne un procédé consistant à obtenir au moins un échantillon contenant des cellules, lequel comprend un acide nucléique contenant des cellules, à obtenir au moins une enzyme catabolique, à obtenir au moins un inhibiteur de nucléases, à préparer un mélange de l'échantillon, de l'enzyme catabolique et de l'inhibiteur de nucléases, à conserver le mélange dans des conditions dans lesquelles l'enzyme catabolique est active, et à agiter le mélange, l'échantillon étant digéré afin de produire un lysat de l'échantillon contenant un acide nucléique.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)