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1. (WO2006088907) NUCLEIC ACID ISOLATION METHODS AND MATERIALS AND DEVICES THEREOF
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2006/088907    International Application No.:    PCT/US2006/005241
Publication Date: 24.08.2006 International Filing Date: 15.02.2006
IPC:
C12P 19/34 (2006.01)
Applicants: UNIVERSITY OF VIRGINIA PATENT FOUNDATION [US/US]; 250 West Main Street, Suite 300, Charlottesville, VA 22902 (US) (For All Designated States Except US).
CAO, Weidong [CN/US]; (US) (For US Only).
FERRANCE, Jerome, P. [US/US]; (US) (For US Only).
LANDERS, James, P., Ph.d. [US/US]; (US) (For US Only)
Inventors: CAO, Weidong; (US).
FERRANCE, Jerome, P.; (US).
LANDERS, James, P., Ph.d.; (US)
Agent: GREENBAUM, Michael, C.; Blank Rome Llp, Suite 1100, 600 New Hampshire Avenue, NW, Washington, DC 20037 (US)
Priority Data:
60/653,203 15.02.2005 US
Title (EN) NUCLEIC ACID ISOLATION METHODS AND MATERIALS AND DEVICES THEREOF
(FR) PROCEDES D'ISOLATION D'ACIDE NUCLEIQUE ET MATERIAUX ET DISPOSITIFS ASSOCIES
Abstract: front page image
(EN)The present invention relates to methods for purifying nucleic acid from a sample using mild conditions that do not affect the chemical integrity of the nucleic acid. The method comprises contacting the sample with an matrix entrapped chitosan solid phase which is able to bind the nucleic acids at a first pH, and then extracting the nucleic acid from the solid phase by using an elution solvent at a second pH.
(FR)L'invention concerne des procédés permettant de purifier un acide nucléique provenant d'un échantillon dans des conditions douces qui n'affectent pas l'intégrité chimique de l'acide nucléique. Le procédé de l'invention consiste à mettre en contact l'échantillon avec une phase solide de chitosane piégée dans une matrice pouvant se lier aux acides nucléiques à un premier pH, puis à extraire l'acide nucléique de la phase solide au moyen d'un solvant d'élution à un deuxième pH.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)